姜黄素对大肠癌细胞Caspase-3和PARP表达的影响

Effects of Curcumin on Caspase-3 and PARP Expression of Colorectal Carcinoma Cells

DOI：10.3969/j.issn.1673-1689.2015.07.013

中文关键词: 姜黄素 Caspase-3 PARP 凋亡 大肠癌

英文关键词: curcumin, Caspase-3, PARP, apoptosis, colorectal cancer

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中文摘要:

为了探讨姜黄素对LoVo细胞凋亡与凋亡相关蛋白Caspase-3和多聚腺苷酸二磷酸核糖聚合酶(PARP)表达的影响，明确其治疗大肠癌的生物学基础。用不同浓度姜黄素处理体外培养的人大肠癌LoVo细胞，采用MTT法检测姜黄素对LoVo细胞的增殖抑制作用，倒置显微镜观察姜黄素对LoVo细胞集落形成的影响；Hoechst 33258染色法观察细胞凋亡；采用分光光度法检测Caspase-3酶活性；Western blotting检测姜黄素作用前后Caspase-3和PARP蛋白表达的差异。结果显示姜黄素可抑制人大肠癌LoVo细胞的生长増殖，并能抑制细胞集落的形成，降低细胞增殖生存率，激活Caspase-3的表达，呈量效和时间依赖性，20 μmol/L以上浓度姜黄素能显著诱导LoVo细胞凋亡，上调Caspase-3和PARP蛋白表达。这表明姜黄素能显著抑制LoVo细胞的增殖并诱导其发生凋亡，其分子机制与调节Caspase-3和PARP的表达相关。

英文摘要:

This study aimed to elucidate the biological basis for the treatment of colorectal cancer using curcumin，by investigating the effects of curcumin on apoptosis of LoVo and the expression of apoptosis-related protein Caspase-3 and poly ADP-ribose polymerase(PARP). The human colorectal carcinoma cells LoVo was conventionally cultured in vitro by curcumin with different concentrations. Methyl thiazolyl tetrazolium(MTT) assays were performed to examine the inhibition of LoVo cell proliferation induced by curcumin. The effect of curcumin on LoVo cells colony formation was observed under an inverted microscope. The fluorescent dye Hoechst 33258 was used to detect the apoptotic cells. The enzyme activity of Caspase-3 was measured using a spectrophotometric method. The levels of Caspase-3 and PAPR expression before and after curcumin treatment were determined with Western blot analysis. The LoVo cells proliferation and the cell colony formation could be inhibited by curcumin. The cell proliferation and survival of LoVo were reduced and the expression of Caspase-3 was activated dependent on the treated concentration and duration. The apoptosis of LoVo cell was significantly induced and the expressions of Caspase-3 and PARP were increased by curcumin with a concentration above 20 μmol/L. The results suggested that curcumin could significantly inhibit LoVo cell proliferation and induce cell apoptosis. The molecular mechanism was associated with the regulation of Caspase-3 and PARP expression.

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