9顺维A酸对人肺腺癌细胞A2增殖及凋亡影响的实验研究

Effects of 9-Cis-Retinoic Acid on the Proliferation and Apoptosis of Human Lung Cancer Cell Line A2

DOI：10.3969/j.issn.1673-1689.2015.07.017

中文关键词: 9-顺维A酸 肺癌 细胞增殖 细胞凋亡

英文关键词: 9-cis-retinoic acid, lung cancer, cell proliferation, apoptosi

基金项目:

摘要点击次数: 179

全文下载次数: 707

中文摘要:

研究9顺维A酸(9-cis RA)抑制肺腺癌细胞A2增殖及诱导凋亡的作用并初步探讨其作用机制。体外培养A2细胞，随机分为4组，实验组加9-cis RA 使其终浓度为1、5、10 μmol/L，对照组加入二甲亚砜使其终质量分数为0.1%，采用MTT法检测各组A2细胞增殖情况；使用流式细胞术(flow cytometry，FCM)分析药物作用后各组细胞的凋亡率；用逆转录-聚合酶链反应(RT-PCR)检测各组细胞bax、fas、bcl-2基因的变化。9顺维A酸对人肺癌细胞株A2增殖均有明显的抑制作用(P

英文摘要:

The study aimed to explore the mechanism of 9-cis-retinoic acid(9-cis RA) inhibiting proliferation and inducing apoptosis in human lung cancer cell line A2.Cultured A2 cells were randomly divided into four groups，the experimental group received 9-cis RA to a final concentration of 1，5 or 10 μmol/L，and the control group was added dimethyl sulfoxide to a final concentration of 0.1%. The MTT method was used for the determination of cell proliferation，flow cytometry(FCM) for the analysis of apoptosis，and reverse transcription-polymerase chain reaction(RT-PCR) for the expression changes of bax，fas and bcl-2 genes. Results showed that 9-cis RA significantly inhibited the cell proliferation(P

查看全文 查看/发表评论 下载PDF阅读器