双向电泳技术在副干酪乳杆菌盐胁迫应激反应研究中的应用

Proteomic Analysis of Protein Induction in Lactobacillus paracasei by Salt Stress Using Two-Dimensional Electrophoresis

DOI：10.3969/j.issn.1673-1689.2015.02.005

中文关键词: 蛋白质组 盐胁迫 乳杆菌 双向电泳

英文关键词: proteomics,salt stress,Lactobacillus paracasei,2-DE

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中文摘要:

对耐盐副干酪乳杆菌在盐胁迫下的应激反应进行研究,比较其在含有6 g/dL NaCl和未含有NaCl的培养基中生长至对数生长中期的蛋白质组双向电泳图谱差异。结果表明:有20个蛋白质点表达发生明显变化,经过基质辅助激光解吸/电离飞行时间质谱鉴定,其中热休克蛋白质GroEL和DnaK在盐胁迫下表达上调,参与脂肪酸合成的3-氧酰基-(酰载体蛋白)合酶Fab G表达下调,可能与该菌应对盐胁迫密切相关。

英文摘要:

Lactobacillus paracasei LN-1 with salt tolerance property was isolated from traditional fermented food,suancai,which was made in the northeast of China. In order to understand the mechanism involved,proteomics analysis was undertaken to reveal the response of the strain during growth in medium with or without NaCl using two-dimensional electrophoresis(2-DE). Out of 20 protein spots that showed differential changes in expression,seven spots were identified by matrix assisted laser desorption and ionization time-of-flight mass spectrometry(MALDI-TOF/MS). Further analysis showed that chaperone protein(GroEL and DnaK) and fatty acid biosynthesis enemy(Fab G) possibly play important role in Lactobacillus paracasei LN-1 to resist to salt stress.

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