骆驼瘤胃乳酸菌对吡啶的降解研究

Study on the Biodegradation of Pyridine by Lactobacillus Reuteri from Camel Rumen Extracts

DOI：10.3969/j.issn.1673-1689.2015.04.004

中文关键词: 关键词:骆驼瘤胃 乳酸菌 吡啶 降解

英文关键词: camel rumen, lactobacillus reuteri, pyridine, biodegradation

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中文摘要:

将来源于骆驼瘤胃中的8株乳酸菌接种到以吡啶(300 μg/mL)为唯一碳源、氮源的无机盐培养基中进行培养,通过紫外分光光度计定期检测乳酸菌的的吸光度值(600 nm),了解其生长情况；利用气相色谱-质谱联用仪(GC-MS)动态检测的乳酸菌降解吡啶的残留量,确定骆驼瘤胃中的乳酸菌对吡啶的降解能力。实验结果显示:8株乳酸菌对吡啶都具有较强的降解能力,其中2株乳酸菌(GU366027、GU366021)培养84 h后,可以将吡啶完全降解；3株菌(GU366034、GU366019、GU366030)96 h后可以100%利用吡啶；3株(GU366037、GU366038、GU366028)108h能够将培养基中的吡啶完全转化。研究提示骆驼瘤胃中的乳酸菌具有高效降解吡啶的作用。

英文摘要:

8 lactobacillus reuteri were isolated from camel rumen and then cultured in mineral saults medium using pyridine (300 μg/mL) as the sole carbon source and nitrogen source. The absorbance at 600 nm was periodically measured by UV spectrophotometer to check the growth of Lactobacillus. The degradation of pyridin by lactobacillus reuteri was dynamically detected using Gas Chromatography-Mass Spectrometry (GC-MS) and the residual amount after degradation was calculated. According to the results, all the lactobacillus could effectively degraded pyridine, among which GU366027 and GU366021 can completely degraded pyridine when cultured for 84 h, while GU366034, GU366019 and U366030 needed 96h and others (GU366037, GU366038, GU366028) for 108 h.

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