西瓜花叶病毒三抗体夹心法与纸质免疫检测传感器检测方法的建立

TAS-ELISA and Electrochemical Paper Assisted Immunosensor for the Detection of Watermelon Mosaic Virus

DOI：10.3969/j.issn.1673-1689.2015.04.005

中文关键词: 西瓜花叶病毒2号 外壳蛋白 三抗体夹心法 电化学纸质免疫检测传感器

英文关键词: watermelon mosaic virus 2, coat protein, TAS-ELISA method, electrochemical paper assisted immunosensor

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中文摘要:

利用西瓜花叶病毒2号外壳蛋白的小鼠单克隆抗体与兔多克隆抗体,建立两种针对西瓜花叶病毒2号的检测方法。运用现有的抗体建立了三抗体夹心酶联免疫吸附法(TAS-ELISA)；运用碳纳米管与抗体包裹滤纸制备纸质免疫检测传感器,再基于电化学工作站建立电化学纸质免疫检测传感器检测方法。两种方法对实际样品检测限分别为0.15、0.20 μg/mL,检测时间分别为3 h和30 min。根据结果分析比较,三抗体夹心法具有较高的检测灵敏度,而电化学纸质免疫传感器检测方法具有更短的检测时间。

英文摘要:

Using a mice monoclonal antibody and a rabbit polyclonal antibody of the watermelon mosaic virus (WMV)-2 coat protein, we developed two detection methods for WMV-2. The trimer antibody sandwich enzyme-linked immunosorbent assay (TAS-ELISA) method was established based on two different antibodies; the electrochemical paper assisted immunosensor detection method was established by combining the electrochemical workstation with the paper assisted immunosensor prepared by the carbon nanotube and the antibody-coated filter paper. The detection limit was 0.15 and 0.2 μg/ml, and the testing time was 3 hours and 30 minutes for two methods, respectively. According to these results, the TAS-ELISA method had a higher detection sensitivity, and the electrochemical paper assisted immunosensor detection method had a shorter testing time.

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