可得然胶生产菌种的筛选及发酵条件优化

Isolation of a Curdlan-Producing Bacterium and Optimization of Fermentation Medium

DOI：10.3969/j.issn.1673-1689.2018.07.010

中文关键词: 可得然胶 正交试验 根瘤菌属 苯胺蓝

英文关键词: curdlan,orthogonal optimization,aniline blue,Rhizobiumsp.

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中文摘要:

采用苯胺蓝平板筛选法从土壤中筛选并鉴定了一株产可得然胶的根瘤菌，并对该菌株发酵合成可得然胶的培养基进行优化。在单因素优化的基础上，设计4因素3水平的正交优化方案，优化结果显示，该菌株发酵产可得然胶的最优培养基组成为：葡萄糖50 g/L，（NH4）2HPO4 2 g/L，KH2PO4 1.5 g/L，MgSO4 1.25 g/L，FeSO4·7H2O 0.05 g/L，MnSO4 0.02 g/L，CoCl2·6H2O 0.01 g/L，ZnCl2 0.01 g/L。经摇瓶发酵验证，可得然胶的产量和糖转化率较优化前分别提高了29.2%和5.4%，为进一步利用该根瘤菌发酵合成可得然胶奠定了良好基础。

英文摘要:

A Rhizobium sp. capable of producing curdlan was isolated from soil. The polysaccharide product was identified to be curdlan by testing the thermal property，molecule composition，infrared spectrum and molecule weight. Furthermore，the orthogonal optimization experiment was conducted to optimize the curdlan fermentation medium，and the best medium composition was 50 g/L glucose，2 g/L（NH4）2HPO4，1.5 g/L KH2PO4，1.25 g/L MgSO4，0.05 g/L FeSO4·7H2O，0.02 g/L MnSO4，0.01 g/L CoCl2·6H2O，0.01 g/L ZnCl2. Consequently，the curdlan production and glucose conversion rate were enhanced by 29.2% and 5.4% compared with those before optimization.

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