改性魔芋葡甘露聚糖对齐口裂腹鱼脂质代谢及相关基因表达的影响

Effect of Modified Konjac Glucomannan on Lipid Metabolism and Related Gene Expression ofSchizothorax prenanti

DOI：10.3969/j.issn.1673-1689.2018.07.014

中文关键词: 改性魔芋葡甘露聚糖 齐口裂腹鱼 ELISA 荧光定量PCR 脂质代谢

英文关键词: modified konjac glucomannan,Schizothorax prenanti,ELISA,real-time quantitative PCR,lipid metabolism

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中文摘要:

对比了两种改性魔芋葡甘露聚糖对齐口裂腹鱼生长性能、肝脏基础成分、血清生化指标及肝脏相关基因表达的影响，采用酶联免疫吸附测定法（ELISA）对齐口裂腹鱼血清高密度脂蛋白胆固醇（HDL-C）、低密度脂蛋白胆固醇（LDL-C）、总胆固醇（T-CHO）及甘油三酯（TG）进行分析，利用实时荧光定量PCR技术对鱼类脂肪合成和沉积过程的关键基因肝脏脂蛋白酯酶（lpl）、肝酯酶（hl）、脂肪合成酶（fas）相对表达量进行定量分析。结果表明：改性魔芋葡甘露聚糖种类、添加剂量以及两者交互水平对齐口裂腹鱼生长均无显著影响。在魔芋葡甘露聚糖硫酸酯（OKGMS）改性条件下，随着添加剂量的增加肝脏脂肪、血清LDL-C、T-CHO、TG含量显著降低，当添加质量分数1.6%OKGMS达到最低；在酸解魔芋葡甘露聚糖改性条件下（AOKGM），随着添加剂量的增加血清LDL-C、T-CHO、TG质量分数呈先降低后增加趋势，当添加质量分数0.8%AOKGM达到最低，均与对照组达到显著差异。与基础组相比，添加质量分数1.6%OKGMS、0.8%AOKGM血清HDL-C显著增加，肝脏lpl mRNA表达量分别增加7.7和2.7倍。

英文摘要:

To study the effect of two modified konjac glucomannan on the growth performance，the liver based ingredients，serum biochemical indicators and lipid metabolism-related gene expression ofSchizothorax prenanti.Schizothorax prenantiserum high-density lipoprotein cholesterol（HDL-C），low-density lipoprotein cholesterol（LDL-C），total cholesterol（T-CHO） and triglyceride（TG） were determined based on enzyme-linked immunosorbent assay（ELISA）.The number of lipoprotein lipase（lpl），hepatic lipase（hl） and fatty acid synthetase（fas） in the liver were quantified by real-time quantitative PCR. The results showed as follows：modified konjac glucomannan type，adding dose and the interaction level had no significant influence on Schizothorax prenanti growth，grossness degree. In konjac glucomannan sulfate（OKGMS） modification conditions，with the increase of adding dose liver fat，serum LDL - C and T - CHO and TG content decreased significantly，when adding 1.6% OKGMS reached the lowest；In Acid oxidation of konjac glucomannan（AOKGM），with the increase of adding dose serum LDL - C，T - CHO and TG content is increased after lower first，when adding 0.8% AOKGM reached the lowest，all groups significant differences with the control group. Compared with the basic group，adding 1.6% OKGMS，0.8% AOKGM significantly increase serum HDL-C level，liver LPL mRNA expression quantity increased by 7.7 and 2.7 times respectively.

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