亚硫酸氢钠对酵母GJ2008高质量浓度蔗糖乙醇发酵的影响

Influence of Sodium Bisulfite on Alcoholic Fermentation from High Gravity Sucrose bySaccharomyces cerevisiaeGJ2008

DOI：10.3969/j.issn.1673-1689.2019.12.019

中文关键词: 亚硫酸氢钠 高浓度蔗糖乙醇发酵 曲线下面积 葡萄糖果糖利用差异性

英文关键词: sodium bisulfite,high gravity ethanol fermentation from sucrose,area under the curve,differences in the glucose and fructose consumption

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中文摘要:

探索不同质量浓度亚硫酸氢钠对酵母GJ2008高质量浓度蔗糖乙醇发酵的影响，旨在为甘蔗糖蜜高质量浓度乙醇发酵提供研究基础。用YPS培养基替代甘蔗糖蜜，调节亚硫酸氢钠质量浓度为0.00、0.16、0.49、1.14、1.63 g/L，以初始酵母数为5.15×107个/mL进行乙醇发酵，采用曲线下面积等方法对发酵过程进行分析。结果表明：亚硫酸氢钠抑制了酵母的活性，使总糖消耗速率变慢和乙醇产率降低；亚硫酸氢钠质量浓度越高，影响越大。当亚硫酸氢钠质量浓度为1.63 g/L时，与对照组相比最大总糖消耗速率降低了53.49%，发酵周期延长了37.50%，乙醇产率下降了33.86%。与果糖代谢相比葡萄糖代谢更易受到亚硫酸氢钠的抑制，且抑制程度随亚硫酸氢钠质量浓度的增大而增强。

英文摘要:

To explore the impact of sodium bisulfite on alcoholic fermentation bySaccharomyces cerevisiae /GJ2008 for acquiring basic information for alcoholic production from high gravity sugarcane molasses. Alcoholic fermentation was carried out by GJ2008 in YPS broth supplemented with sodium bisulfite of 0.00，0.14，0.49，1.14，1.63 g/L，respectively and with an initial yeast number of 5.15×107 cells/mL. Area under the curve（AUC）and other method was adopted to analyze the process of alcohol fermentation. The results showed that sodium bisulfite inhibited the vitality of yeast cell and slowed down the rate of sugar uptake with reduced the ethanol productivity. The higher concentration of sodium bisulfite was，the greater influence was. Compared to the control sample（no sodium bisulfite added），the total sugar uptake rate of the maximum decreased by 53.49%，fermentation time extended by 37.50% and ethanol productivity reduced by 33.86% when the concentration of sodium bisulfite increased up to about 1.63 g/L. The results also showed that glucose utilization was inhibited to a larger extent than fructose by sodium bisulfite，whose inhibition extent to sugars was gradually enhanced with the increase of the concentration of sodium bisulfite.

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