草苁蓉环烯醚萜苷对肝癌细胞凋亡及Bax和Bcl-2蛋白表达的影响

Effect of Iridoid Glycosides fromBoschniakia rossicaon Apoptosis and Expression of Bax and Bcl-2 in Liver Cancer Cells

DOI：10.3969/j.issn.1673-1689.2019.12.020

中文关键词: 肝癌 草苁蓉环烯醚萜苷 细胞凋亡 Bax Bcl-2

英文关键词: liver cancer,Iridoid glycosides fromBoschniakia rossica,apoptosis,Bax,Bcl-2

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中文摘要:

观察二乙基亚硝胺（DEN）诱发大鼠肝癌组织的病理变化，探讨草苁蓉环烯醚萜苷（IGBR）对细胞凋亡和Bax、Bcl-2蛋白表达的影响。将Wistar大鼠分为对照组、模型组、5-FU组及IGBR组。除对照组，各组大鼠第1天给予200 mg/kg DEN腹腔注射1次，而后给予饮用体积分数0.05%的DEN水溶液；5-FU组大鼠腹腔注射25 mg/kg 5-FU每周3次；IGBR组大鼠灌胃500 mg/kg IGBR每日1次。实验第12、20、28周末，分批处死动物，观察肝脏病理变化及细胞凋亡，免疫组化法检测Bax、Bcl-2蛋白的表达。结果表明，与对照组比较，模型组细胞凋亡显著增加，胞质呈棕黄色，凋亡的肝细胞皱缩、核固缩及核仁消失；与模型组比较，5-FU组与IGBR组凋亡显著增多，差异有统计学意义（PP>0.05）；Bax和Bcl-2阳性细胞主要分布于不典型增生灶和癌灶中，表达于胞浆；与模型组比较，5-FU组和IGBR组Bax表达明显增强，而Bcl-2表达明显减弱，差异有统计学意义（PPP>0.05）；模型组、5-FU组与IGBR组中Bax表达和Bcl-2表达均呈负相关（P

英文摘要:

Observation of diethyl nitrosamine（DEN） induced rat liver cancer tissue pathological changes，discussesBoschniakia rossicairidoid glycoside（IGBR） on apoptosis the influence of Bax， Bcl-2 protein expression.Wistar male rats were randomly divided into control group，model group，5-FU group and IGBR group. were injected intraperitoneally once a day for 200 mg / kg DEN intra peritoneally on the 1st day，Rats in the control group were given equal volume of saline；then given free access to 0.05% DEN solution for up to 28 weeks. The IGBR group was gavaged 500 mg/kg IGBR once dailyk，and 5-FU group was injected with 25 mg/kg 5-FU 3 times a wee. The animals were sacrificed in batches after 12，20 or 28 weeks of DEN exposure，and the pathological changes and apoptosis of liver were observed. The expression of Bax and Bcl-2 were detected with the immunohistochemical method.The results showed that，compared with the control group，the cell apoptosis increased significantly，and the cytoplasm was brownish yellow. Apoptotic hepatocyte showed cytoplasmic and nuclear shrinkage，disappearance of nucleolus. The apoptotic of the 5-FU and IGBR groups were higher than those of model group（PP>0.05）.The Bax and Bcl-2 positive cells were mainly distributed in the atypical hyperplasia and neoplastci foci. In the 5-FU and IGBR groups，the expression of Bax was up-regulated and Bcl-2 was down-regulated as compared with the model group（PP>0.05）. The expression of Bax and Bcl-2 in the model group，5-FU group and IGBR group were negatively correlated. IGBR can induce apoptosis，Inhibit the proliferation of liver cancer cells Its mechanism is likely related to the up-regulation of Bax and down-regulation of Bcl-2．

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