喷雾干燥对德氏乳杆菌代谢酶活与可培养性的影响

Effects of Spray Drying on Metabolic Enzyme Activity and Culturability ofLactobacillus delbrueckii

DOI：10.3969/j.issn.1673-1689.2019.11.016

中文关键词: 喷雾干燥 代谢酶活 产酸性能 可培养性

英文关键词: spray drying,metabolic enzyme activity,acid-producting capacity,culturability

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中文摘要:

为了提高乳酸菌在喷雾干燥过程中的可培养性，以德氏乳杆菌ATx为研究对象，在分析不同热胁迫条件对其细胞可培养性的影响的基础上，以20 g/dL脱脂乳（SM）、12 g/dL脱脂乳+8 g/dL菊糖（SMS）、12 g/dL脱脂乳+8 g/dL海藻糖（SMT）、12 g/dL脱脂乳+4 g/dL菊糖+4 g/dL海藻糖（SMST）为保护剂，探讨了喷雾干燥对菌体己糖激酶、乳酸脱氢酶、细胞膜ATP酶、细胞可培养性以及产酸性能的影响。结果表明：德氏乳杆菌ATx的最佳热胁迫条件为（50 ℃，15 min），此时细胞活菌数仅下降0.107 lg CFU/mL（P≥0.05）。经过喷雾干燥后菌体的细胞膜ATP酶、已糖激酶与乳酸脱氢酶的活性均显著地降低（P

英文摘要:

In order to improve the culturability of lactic acid bacteria during spray drying ，taking Lactobacillus delbrueckii（ATx） as study objects，culturability of lactic acid bacteria have been investigated after heat stress. And the effects of spray drying on the activities of hexokinase，lactate dehydrogenase，cell membrane ATPase，cell viability and acid-producting capacity have been investigated in four kinds of protective agent（20% skim milk（SM），12% skim milk +8% synanthrin（ SMS），12%skim milk +8% trehalose（SMT），12% skim milk+4% synanthrin +4% trehalose（SMST））. The results showed that the optimal heat stress condition of ATx was（50 ℃，15 min），and the number of viable cells only decreased by 0.107 lg CFU / mL（P≥ 0.05）. It was worth mentioning that after spray drying，the activities of ATPase，carbohydrolase and lactate dehydrogenase were significantly decreased（P

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