纳滤脱盐在ε-聚赖氨酸分离提取中的应用

Application of Nanofiltration in the Separation and Purification ofε-Poly-L-Lysine for Desalination

DOI：10.3969/j.issn.1673-1689.2019.11.017

中文关键词: ε-聚赖氨酸 纳滤 脱盐 渗滤

英文关键词: ε-poly-L-lysine,nanofiltration,desalination,diafiltration

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中文摘要:

为了建立ε-聚赖氨酸（ε-PL）分离提取过程中的纳滤脱盐方法，作者在纳滤膜种类筛选基础上，以模拟料液为研究对象，通过单因素实验优化了纳滤膜脱盐条件，并将其应用到离子交换洗脱液的脱盐中。研究结果表明，纳滤膜SP800在透过通量、ε-PL收率和脱盐率等指标上均最优，适合用于ε-PL脱盐；纳滤膜SP800脱盐条件为：料液pH值为9.0，最大浓缩倍数对应ε-PL质量浓度为100 g/L左右，再加入去离子水以恒容渗滤方式脱盐，直到透过液电导率低于300 μS/cm为止。该脱盐工艺处理1 000 L ε-PL离子交换洗脱液时，脱盐率达到96.43%，ε-PL损失率0.77%；ε-PL纯度达到98.21%，灰分为1.02%。本研究是首次尝试纳滤用于ε-PL分离和提取过程脱盐，对ε-PL产业化生产具有一定的指导意义。

英文摘要:

To develop the process of desalination in the separation and purification of ε-poly-L-lysine（ε-PL），the nanofiltration was used in this study. Firstly，several nanofiltration membranes were evaluated，，then the operation parameters were optimized through one-factor-at-a-time experiment using model solution as object. Finally，the eluent of ion exchange step was desalinated by nanofiltration at the optimal condition. The results showed that SP800 was the best choice used for desalination in terms of permeate flux，ε-PL retention ratio and NaCl removal ratio. Meanwhile，the optimal desalination conditions were as follows：the pH was 9.0，and the maximum ε-PL concentration was concentrated to about 100 g/L，while the permeate conductivity was controlled less than 300 μS/cm through adding deionized water. To evaluate the efficient of the developed desalination process，1 000 L ε-PL eluent of ion exchange step was employed for desalination by nanofiltration at the optimal condition. Finally，the NaCl removal ratio and the ε-PL loss ratio were reached 96.43% and 0.77%，respectively；and the ε-PL purity and ash content were attained 98.21% and 1.02%，respectively. This is the first report on the desalination of ε-PL solution using nanofiltration，it would be helpful to the production of ε-PL in industry.

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