猴头菌粉对结肠炎小鼠肠道菌群的影响

Effect ofHericium erinaceuson Gut Microbiota in Mice with Colitis

DOI：10.3969/j.issn.1673-1689.2019.09.014

中文关键词: 猴头菌 结肠炎 肠道菌群 变形梯度凝胶电泳

英文关键词: Hericium erinaceus,colitis,gut microbiota,PCR-DGGE

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中文摘要:

探究猴头菌粉对小鼠结肠炎的治疗效果及肠道菌群的影响。C57BL/6雄性小鼠随机分为正常组、DSS模型组、猴头菌粉低剂量组和猴头菌粉高剂量组，采用质量分数2%葡聚糖硫酸钠（Dextran Sulfate Sodium，DSS）诱导C57BL/6小鼠建立结肠炎动物模型，低剂量组（250 mg/kg/d）和高剂量组（500 mg/kg/d）小鼠同时给予猴头菌粉和质量分数2% DSS，7 d后进行疾病活动指数评分和病理组织切片观察，并利用变性梯度凝胶电泳技术分析小鼠肠道微生物的变化。结果：猴头菌粉在一定剂量（250 mg/kg/d和500 mg/kg/d）下均表现出良好的抗结肠炎效果，其摄入能显著增加结肠炎小鼠体重、降低疾病活动指数评分并改善结肠组织病理形态；抑制由DSS造成的肠道菌群紊乱，增加了Akkermansia muciniphila和Barnesiella intestinihominis的比例。研究结果提示猴头菌粉可能通过调节肠道微生物比例，改善小鼠结肠炎。

英文摘要:

The study aimed to investigate the effect ofHericium erinaceus（HE） on intestinal microbiota of mice with colitis. C57BL/6 male mice were randomly divided into four groups （normal，model，HE low and high dose groups）. Mice were induced by 2% dextran sulfate sodium （DSS） to establish the mouse model of ulcerative colitis. Meanwhile，mice of high and low dose groups administrated dry powder of HE （250 mg/kg/d and 500 mg/kg/d） and drunk 2% DSS. After 7 days，disease activity index was scored and the shift of gut microbiota was examined by PCR-DGGE technology. The results showed that HE possesses anti-colitis activity. The administration of HE could significantly increase the body weight，decrease the score of disease activity index，and improve the histological change induced by DSS. Furthermore，HE restored the disordered gut microbiota caused by DSS and increased the proportions ofAkkermansia muciniphilaandBarnesiella intestinihominis. Therefore，the results demonstrated that HE possesses anti-colitis activity，partially through modulating gut microbiota.

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