大孔树脂分离纯化横山老黑豆酚类物质

Separation and Purification of Polyphenol in Hengshan Black Soybean by Macroporous Resin

DOI：10.3969/j.issn.1673-1689.2019.09.015

中文关键词: 横山老黑豆 酚类化合物 大孔树脂 分离纯化

英文关键词: Hengshan black soybean,phenolic compound,macroporous resin,separating and purifying

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中文摘要:

比较8种大孔树脂对横山老黑豆酚类化合物的静态吸附和解吸特性，筛选出分离最佳的大孔树脂，分析静态吸附动力学曲线和最佳的乙醇解吸体积分数，优化不同的样品质量浓度和不同上样流速动态吸附工艺参数。HPLC方法确定8种酚酸含量以及5种异黄酮含量。结果表明，NKA-9大孔树脂的静态吸附和解吸对横山老黑豆酚类化合物的效果最好，静态吸附4 h可达到平衡，乙醇解吸体积分数为70%。动态吸附和解吸参数：样品上样质量质量浓度为1.96 mg/mL，上样流速为2.14 BV/h。HPLC分析和鉴定NKA-9大孔树脂分离纯化前后的横山老黑豆酚类物质种类没有变化，含量降低很少。横山老黑豆酚类物质中原儿茶酸、绿原酸、咖啡酸、大豆苷和染料木苷含量较高。

英文摘要:

The optimum separating macroporous resin were selected through comparing static adsorption and desorption characteristics of eight macroporous resins for the total phenols of Hengshan black soybean. Analysing absorption kinetic curve and ethanol desorption concentration，different samples concentration and flow rate were optimized. HPLC was used to determine the contents of phenol acids and isoflavones in Hengshan black soybean. The results showed that NKA-9 macroporous resin possess the best capability of adsorption and desorption. The macroporous resin was in equilibrium within 4h. The optimum separation conditions were as follows：eluent 70% ethanol，sample concentration 1.96 mg/mL，flow rate 2.14 BV/h. HPLC method indicated that the categories of polyphenols in Hengshan black soybean were not changed after adsorption and desorption experiments by NKA-9 macroporous resin，the content decreased a little. The contents of protocatechuic acid，chlorogenic acid，caffeic acid，daidzin and genistin were higher.

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