产耐热β-半乳糖苷酶蜜源芽孢杆菌的鉴定及酶学性质

Identification of Thermostable β-Galactosidase-ProducingBacillusStrain from Honey and Enzymatic Properties

DOI：10.3969/j.issn.1673-1689.2019.07.007

中文关键词: 关键字： β-半乳糖苷酶 酶学性质 地衣芽孢杆菌

英文关键词: Keyword： β-galactosidase,enzymatic properties,Bacillus licheniformis

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中文摘要:

从分离自蜂蜜的芽孢杆菌中筛选到一株高产β-半乳糖苷酶的菌株，鉴定为地衣芽孢杆菌（Bacillus licheniformis），并命名为Bacillus licheniformisSYBC hb15。纯化该酶并研究其酶学性质，以邻硝基酚-β-D-半乳糖苷（ONPG）为底物，B. licheniformis SYBC hb15所产β-半乳糖苷酶的最适水解反应温度是60 ℃；70 ℃放置60 min后酶活力仍保留65%，具有较高的热稳定性；与嗜热菌Talaromyces thermophilus来源的β-半乳糖苷酶相比，葡萄糖对其水解活性的抑制较弱。因此，B. licheniformisSYBC hb15所产的β-半乳糖苷酶具有较高的热稳定性和葡萄糖耐受性，有很好的工业应用潜力。

英文摘要:

A β-galactosidase-producing strain was screened from bacillus species isolatated from honey. The strain was identified asBacillus licheniformisby an in-depth study and named asBacillus licheniformisSYBC hb15. β-galactosidase expressed byBacillus licheniformisSYBC hb15 was purified for the study of enzymatic properties using o-nitrophenyl-β-D-galactoside（ONPG） as substrate. The result showed that the optimal reactive temperature of the β-galactosidase was 60 ℃，and it had high thermal stability when incubating the enzyme at 70 ℃ for 60 min with 65% residual activity. Compared to that of the enzyme from Talaromyces thermophilus，glucose presents less inhibition to the hydrolytic activity of the β-galactosidase. Therefore，the β-galactosidase produced byB. licheniformisSYBC hb15 has good potential in the industrial application because of its high thermostability and resistance to glucose of high levels.

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