活性氧对白腐真菌漆酶合成的影响

Effect of Reactive Oxygen Species on Laccase Synthesis of White-Rot Fungus

DOI：10.3969/j.issn.1673-1689.2019.05.015

中文关键词: 漆酶 活性氧 白腐真菌

英文关键词: laccase,reactive oxygen species,white-rot fungi

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中文摘要:

分别将不同活性氧在发酵第4天加入发酵产酶培养基中，一定时间后通过测生物量和漆酶酶活研究外界活性氧对白腐真菌漆酶合成的影响，并通过活性电泳进一步验证。结果表明：低浓度的活性氧可以通过信号传导方式调控漆酶合成，超氧阴离子抑制白腐真菌产漆酶，过氧化氢和羟自由基促进白腐真菌产漆酶。实验结果对漆酶合成调控、植物-病原菌相互作用以及生物防护具有重要意义。

英文摘要:

In this paper，various of reactive oxygen species were respectively added to the fermentation medium on the 4 d. After a certain time，the effection of different reactive oxygen species on laccase synthesis of white-rot fungus，was evaluated by measuring biomass and laccase activity. And then this effection was further investigated by the method of native PAGE. The experiment results show that reactive oxygen species can regulate laccase synthesis of white-rot fungus via redox regulation in cellular signaling. In addition，superoxide anion inhibits laccase synthesis，but hydrogen peroxide and hydroxyl radical promote it obviously. These results are very important for researching biosynthesis regulation of laccase and interaction between plant and pathogen，which can be used for biological protection.

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