重组Cystatin对冷藏鲢鱼的抑菌效果

Study on Bacteriostasis of the Cystatin from Chilled Silver Carp

DOI：10.3969/j.issn.1673-1689.2019.05.016

中文关键词: 鲢鱼 4 ℃有氧冷藏 优势假单胞菌 分离鉴定 Cystatin 抑菌作用

英文关键词: silver crap,4 ℃ chilled fillet,spoilagePseudomonas,isolation and identification,Cystatin,antibacterial activity

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中文摘要:

分离鉴定4 ℃有氧冷藏鲢鱼去皮肉片货架期终点腐败相关优势假单胞菌，并研究鲢鱼重组Cystatin（家族II）对优势腐败假单胞菌的抑菌效果。首先利用CFC培养基从4 ℃有氧冷藏鲢鱼去皮肉片货架期终点，分离筛选出腐败相关假单胞菌，并采用生理生化和16 SrDNA法对其进行鉴定，其次将腐败菌株进行回接试验，通过测定各腐败菌回接组的挥发性盐基氮（TVB-N）值、菌落数、感官评分判断其致腐能力。最后，采用滤纸片扩散法和营养肉汤法鉴定重组鲢鱼Cystatin（家族II）对优势腐败假单胞菌的抑菌效果。结果表明，荧光假单胞菌（Pseudomonas fluorescens）和草莓假单胞菌（Pseudomonas fragi）为主要致腐菌，所占比例分别为63.3%、26.7%，且荧光假单胞菌致腐能力强于草莓假单胞菌。重组Cystatin对两种菌均有明显的抑菌效果，且呈剂量依赖关系。

英文摘要:

Investigation on the dominant spoilage pseudomonas from the chilled silver carp filets at the end of shelf life was carried out and then antibacterial experiment were conducted to determine the antibacterial activities of cystatin to the identified dominant spoilage pseudomonas. Firstly，the dominant spoilagePseudomonaswere isolated at the end of shelf life from the chilled silver carp fillets storaged at 4 ℃.Subsequently，physiological and biochemical method combined with 16S rRNA genes were conducted to identify these spoilage pseudomonas.After that，these strains were inoculated back to chilled silver carp filets at 4 ℃ and aerobic condition. TVB-N，the total number of bacterial colony combined with sensory evaluation were measured to evaluate the putrefying ability of these strains. Moreover，the experiments of filter paper diffusion and nutrient broth was both conducted to determine the antibacterial activities of cystatin to the identified dominant spoilage pseudomonas. The results in the present study demonstrated that the main putrefying strains were pseudomonas fluorescens and Pseudomonas fragi corresponding to the percent of 63.3% and 26.7% respectively.Pseudomonas fluorescensowned the stronger putrefying ability than Pseudomonas fragi. The antibacterial activity of the cystatin to the two dominant putrefying pseudomonas was dose-dependent.

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