黄茶“中黄2号”的亚细胞结构透射电镜观察

Transmission Electron Microscopic Study of Subcellular tructureof Yellow Tea Cultivar ‘Zhonghuang 2’

DOI：10.3969/j.issn.1673-1689.2017.12.003

中文关键词: 茶树 黄化变异品种 中黄2号 叶绿体 透射电镜

英文关键词: tea plant,Yellow tea cultivar,Zhonghuang 2,chlorophyll,transmission electron microscope

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中文摘要:

利用透射电镜技术研究了黄化品种“中黄2号”与正常品种“龙井43”第2、6叶的亚细胞结构差异。结果发现中黄2号与龙井43在细胞核、胞质、线粒体、高尔基体等亚细胞结构和分布规律方面基本一致，而叶绿体结构则有较大差异。中黄2号第2叶叶绿体明显偏小，且基粒片层堆叠少于龙井43。其第6叶片层堆叠多于第二叶，但仍少于龙井43。叶绿体内片层堆叠情况与其含量测定结果一致。同时，遮荫处理后中黄2号叶色变绿，叶绿体片层堆叠也增多。研究发现，中黄2号的黄化可能与其叶绿体基粒片层合成受阻有关，该结果为深入解析中黄2号黄化的机理打下基础。

英文摘要:

The subcellular structures of the second and sixth leaves of yellow tea cultivar "Zhonghuang 2" and normal green tea cultivar "Longjing 43" were studied by transmission electron microscope. It was found there were no significant differences in ultrastructure and distribution of nucleus，cytosol，mitochondrion and golgi apparatus. The largest difference was existed in chloroplast ultrastructure. Compared to Longjing 43，smaller chloroplasts and less grana lamellae were found in Zhonghuang 2. The chloroplast lamellae numbers were increased in the sixth leaves of Zhonghuang 2，but were still less than those in Longjing 43. The chloroplast lamellae numbers were consistent with the chlorophyll contents determined. Furthermore，the second leaves of Zhonghuang 2 turned green and the chloroplast lamellae numbers increased under shading treatment. In summary，it was the first finding that the yellow tea cultivar "Zhonghuang 2" was correlated with the biosynthesis of chloroplast lamellae，which will also be a basis for further understanding its yellowing mechanism in Zhonghuang 2.

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