小麦麸皮木聚糖制备方法的研究

Research on a Method of Xylan Preparation from Wheat Bran

DOI：10.3969/j.issn.1673-1689.2017.10.018

中文关键词: 小麦麸皮 淀粉 粒径 纤维素酶 蛋白质 木聚糖

英文关键词: wheat bran,starch,particle size,cellulose,protein,xylan

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中文摘要:

研究了小麦麸皮木聚糖的制备，借助高速粉碎处理的方法分离麸皮中淀粉，再采用胶体磨与纤维素酶处理及碱浸泡处理，回收蛋白质，最后通过优化确定最佳低聚糖的最佳工艺。试验结果显示粉碎时间2 min，试验原料的粒径范围为>125 μm，淀粉得率72.43%；纤维素酶和碱处理的最佳工艺条件为：加酶量1.0 mL/hg，碱处理温度为60 ℃，碱处理pH为9.5，在此条件下木聚糖质量分数为35.5%，蛋白质提取率为74.9%，水洗的最佳水洗次数为4次，此时木聚糖质量分数为36.5%。

英文摘要:

This experiment mainly study the preparation of xylan from wheat bran. First of all，with the help of high speed grinding processing method to separate starch from the bran，then treated with cellulase and alkali soaking for recovering protein，finally by optimizing the optimum procedure to determine the best process for xylan. Experiment results show that the grinding time is 2 min，raw material particle size is in the range of >125 μm，starch yield is 72.43%；Optimum process conditions of cellulase and alkali treatment were that for：with enzyme was 1 mL/100 g bran，alkali treatment temperature was 60 ℃，alkali treatment was pH 9.5，under the condition of the xylan content is 35.5%，protein extraction rate was 74.9%.The best washing number is：4 times，the content of xylan under the condition is：36.5%.

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