自然水体生物膜胞外聚合物的提取及优化

Extraction of Extracellular Polymers from Natural Biofilms

DOI：10.3969/j.issn.1673-1689.2017.05.009

中文关键词: 关键字： 自然水体生物膜 胞外聚合物 提取

英文关键词: natural biofilms,extracellular polymeric substances,extraction

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中文摘要:

以自然水体生物膜为研究对象，蛋白质和多糖的提取量作为生物膜胞外聚合物（EPS）提取总量的衡量指标，核酸含量用于表征细胞破裂程度，本文作者以高速离心法为对照，分别研究了加热法、EDTA法、NaOH+NaCl法和超声+CER法对自然水体生物膜胞外聚合物的提取效果，从而确定自然水体生物膜最适提取方法并对实验条件进行优化。研究结果表明，NaOH+NaCl法因提取胞外聚合物总量高，核酸含量较少为最适提取方法。在NaOH浓度为2 mol/L，NaCl质量分数为0.85%，萃取2.5 h，分离转速为7 000 g条件下，EPS提取量为157.57 mg/L。

英文摘要:

Using the content of protein and polysaccharide and the content of nucleic acid separately as indicators of total extracellular polymeric substances（EPS） and cell autolysis，five different extraction methods，including thermal，EDTA，NaOH+NaCl，ultrasonic+CER and high speed centrifugation（control） treatments，for EPS from natural biofilms were studied. Results showed that NaOH+NaCl treatment was the most effective for EPS extraction with less nucleic acid contamination. The optimal extraction conditions were 2 mol/L NaOH，0.85% NaCl，extraction time 2.5 h and centrifugal force 7 000 g. With these parameters，the extraction content of EPS was 157.57 mg/L.

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