Aspergillus ficuum产果聚糖酶体系的分析

Analysis of Fructanohydrolase System in Aspergillus ficuum

DOI：

中文关键词: 果聚糖酶,外切菊粉酶,内切菊粉酶,活性聚丙烯酰胺凝胶电泳

英文关键词: fructanohydrolase,exoinulinase,endoinulinase,native PAGE

基金项目:

王静 金征宇

江南大学食品学院 江苏无锡214036

(王静)

，江南大学食品学院 江苏无锡214036(金征宇)

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中文摘要:

采用活性聚丙烯酰胺凝胶电泳法对Aspergillusficuum产果聚糖酶体系进行了分离 ,获得8条谱带 ;进一步运用薄层色谱 (TLC)和高效液相色谱 (HPLC)法进行分析 ,发现 8条谱带中有 3条属于外切菊粉酶 ,2条属于内切菊粉酶 ,证明了Aspergillusficuum能同时产内切菊粉酶和外切菊粉酶 .

英文摘要:

Aspergillus ficuum fructanohydrolases were separated by native polyacrylamide gel electrophoresis. Eight protein bands were obtained. Proved using TLC and HPLC, three bands had exoinulinase activity and two bands had endoinulinase activity.

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