土壤杆菌中辅酶Q10的提取工艺

A Study on Technique for Extracting Coenzyme Q10 from Agrobacterium

DOI：10.3969/j.issn.1673-1689.2009.06.017

中文关键词: 辅酶Q10 农杆菌 提取工艺 中试

英文关键词: Coenzyme Q10 Agrobacterium Extracting industrial production

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中文摘要:

研究了溶剂种类、浓度,提取溶剂比例、提取次数、时间、温度,萃取次数与温度,以及柱纯化和重结晶等条件对提取农杆菌中辅酶Q10的影响;进行了最佳工艺优化,确立了生产工艺,并进行了中试验证。结果表明,最佳工艺条件为:用5倍的体积分数95%的乙醇在50℃浸提2遍,每遍1 h,获得提取液;提取液浓缩后经过提取液-甲醇-石油醚(三者体积比为1∶2∶3)的萃取体系室温萃取3遍,浓缩石油醚相后经过1步硅胶层析柱纯化,2或3步乙醇重结晶。中试结果表明,该工艺获得辅酶Q10的纯度>99%,平均回收率>80%。

英文摘要:

The process parameters of extraction CoQ10 from Agrobacterium were carefully investigated and then validated by pilot-scale test in this manuscript.The optimum process parameters were decided as follows: firstly,extracting twice(one hour each time) using 5X 95%enthanol at 50 ℃ followed by a concentrated step then extracting three times at room temperature using a extraction system ratio of 1∶2∶3(concentrated solution∶methanol∶Petroleum ether) and finally concentrating Petroleum ether phase,through one step of Silica Gel-Alumina Column purification,two to three steps of recrystalization by enthanol to obtain the aim product.The pilot-scale test shown the process gives a purity of over 99%,percentage recovery over 80%.

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