| 去除黄曲霉毒素B_1的菌株筛选
| Screening of Aflatoxin B1 Removal Strains
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| DOI：
| 中文关键词: 黄曲霉毒素B1 去除 降解 巨大芽孢杆菌
| 英文关键词: Aflatoxin B1 removal degradation Bacillus megaterium
| 基金项目:国家科技支撑计划项目(2009BADB9B08)
| 作者
| 单位
| 孙丰芹
| 江南大学,食品学院,江苏,无锡,214122
| 金青哲
| 江南大学,食品学院,江苏,无锡,214122
食品科学与技术国家重点实验室,江南大学,江苏
| 王兴国
| 江南大学,食品学院,江苏,无锡,214122
食品科学与技术国家重点实验室,江南大学,江苏
| 王珊珊
| 山东鲁花集团有限公司,山东,烟台,265200
| 李秋
| 山东鲁花集团有限公司,山东,烟台,265200
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| 中文摘要:
| 作者从花生土壤和花生粕中筛选能去除黄曲霉毒素B1(AFB1)的菌。利用营养特异性方法进行去除AFB1菌株的初筛,之后将初筛的7株菌的细胞和发酵上清液作用于质量浓度为200μg/L的AFB1。结果表明:筛选得到的TRS-3菌株,其活细胞和死细胞对AFB1的去除率分别达到48.26%和53.56%,其发酵上清液降解AFB1的能力达到78.55%,均明显高于其它菌株。通过对TRS-3菌株的鉴定,最终确定为巨大芽孢杆菌(Bacillus megaterium)。
| 英文摘要:
| The objective of this study was mainly to screen the bacteria that could remove aflatoxin B1 in the peanut soil and moldy meal.After the first screening by the nutrient-specific method,the effect of cells and the supernatant fluid of the seven strains on aflatoxin B1(200 μg/L) was investigated.The result demonstrated that among the seven strains,the strain TRS-3 can remove aflatoxin B1 effectively,with 48.26% and 53.56% of the removal ratio of AFB1 by viable and dead cells,respectively.The degradation ratio of the supernatant fluid reached 78.55%,which was significantly higher than that of others.The strain TRS-3 was finally identified as Bacillus megaterium.
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