强力霉素人工抗原的合成与抗体制备
Artificial Antigen Synthesis and Antibody Preparation of Doxycycline
DOI：
中文关键词: 强力霉素 人工抗原 抗体 合成 制备
英文关键词: doxycycline artificial antigen antibody synthesis preparation
基金项目:农业部公益性行业专项项目(200803013)
作者
单位
甘金华
中国水产科学研究院,长江水产研究所,湖北,荆州,434000
邓薇
李进平
武汉纺织大学,环境与城建学院,湖北,武汉,430073
艾晓辉
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中文摘要:
采用改进的碳二亚胺两步法将强力霉素半抗原与载体蛋白BSA连接制备强力霉素-牛血清白蛋白(BSA-DC)人工免疫抗原,并用同样方法将强力霉素与载体蛋白OVA连接制备人强力霉素-卵清白蛋白(OVA-DC)人工包被抗原。经紫外扫描分析和动物免疫试验证实:强力霉素人工抗原合成成功,强力霉素与BSA的结合比为3∶1,经动物免疫试验所得抗血清效价为2.048×106,完全能够满足强力霉素残留的ELISA检测要求。
英文摘要:
In this study,the improved two-step carbodiimide method was used to connecte doxycycline hapten with carrier protein BSA,and doxycycline with the carrier protein OVA to prepare the artificial antigen of doxycycline(BSA-DC) and coating antigen(OVA-DC),respectively.UV scanning analysis and animal immune tests confirm the success in preparation of artificial antigen,and the combination ratio of doxycycline and BSA is 3∶1 by calculating.At the same time,the results showed that high titers of 2.048×106 anti-DC serum were produced by BSA-DC immunized rabbit.It provided a necessary condition for ELISA detecting doxycycline residues.
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