| 棘孢木霉胞外β-糖苷水解酶的产生条件及基本特征
| Production and Characterization of Extracellular β-glycoside Hydrolase from Trichderma Asperellum W03
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| DOI：
| 中文关键词: 木聚糖酶 CMC酶 几丁质酶 生物防治
| 英文关键词: xylanase CMCase chitinase biocontrol
| 基金项目:江西省教育厅科技项目(GJJ08309)
| 作者
| 单位
| 王剑锋
| 东华理工大学,生物系,抚州,344000
| 李江
| 东华理工大学,生物系,抚州,344000
| 杨红
| 东华理工大学,生物系,抚州,344000
| 谭可
| 东华理工大学,生物系,抚州,344000
| 张浩
| 东华理工大学,生物系,抚州,344000
| 刘亚洁
| 东华理工大学,生物系,抚州,344000
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| 中文摘要:
| 分离自煤炭样品的菌株Trichderma asperellumW03在液态培养条件下能同时分泌产生3种酶学性质相近的β-糖苷水解酶:木聚糖酶、羧甲基纤维素酶和几丁质酶,3种酶的最适反应温度分别为45、45和40℃,最适反应pH值为4.5并且在pH值2.5~5.0的条件下酶活稳定;3种活性酶的合成与菌体生长偶联,对碳源、氮源、KH2PO4、MgSO4、CaCl2、pH值和发酵时间等培养因素的响应趋势一致。优化的产酶培养基为(g/L):土豆200、羧甲基纤维素钠2.0、葡萄糖5.0、KNO31.0、蛋白胨1.0、KH2PO41.0、MgSO4.7H2O 0.5、pH 6.0;在33℃、180 r/min和50 mL/250mL的摇瓶培养条件下发酵72 h,木聚糖酶、CMC酶和几丁质酶的酶活分别达到87u/mL,58u/mL和30 u/mL,该菌株在生物防治和饲料加工方面具有潜在应用价值。
| 英文摘要:
| The strain of Trichderma asperellum W03 was isolated from the peat and exhibited the potential to produce xylanase,CMCase and chitinase under the submerged culture.Those three kinds enzymes achived at a highest activity at the conditions: pH 4.5,temperature 40 ℃-45 ℃ and acidic condition.Moreover,the optimum nutrient and culture condistions were determined and listed as follows: 200.00 potato,2.0 sodium carboxymethyl cellulose,5.0 glucose,1.0 KNO3,1.0 peptone,1.0 KH2PO4,0.5 MgSO4·7H2O and pH 6.0.with the optimum conditions,xylanase,CMCase and chitinase reached at 87u/mL,58 u/mL and 30 u/mL,respectively,after 72 h with 33 ℃ and 180 r/min.
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