鳕鱼多肽的抗氧化活性及其分离纯化

Antioxidant Activity and Purification of Pollock Peptides

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中文关键词: 鳕鱼多肽 抗氧化活性 分离纯化

英文关键词: pollock peptides antioxidant activity separation and purification

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中文摘要:

研究了鳕鱼多肽的抗氧化活性及分离纯化。为获得高纯度的抗氧化肽,将鳕鱼多肽依次通过超滤、凝胶过滤层析、阴离子交换层析(Q-FF)和反相高效液相色谱层析(RP-HPLC)进行分离纯化。结果表明:鳕鱼多肽具有很强的清除羟自由基的能力、清除DPPH的能力和还原能力;经Q-FF柱层析分离得到了B1、B2和B33个组分,B2经RP-HPLC检测显示为单一峰,获得了纯度较高的鳕鱼多肽,这为鳕鱼多肽的开发利用提供了科学理论依据。

英文摘要:

The antioxidant activities of Pollock peptides were studied at first in this manuscript and demonstrated he Pollock peptides had strong scavenging hydroxyl free radical activity,and scavenging 1,diphenyl-2-picrylhydrazyl(DPPH) activity,and reducing activity.In order to obtain high homogenous antipeptide,the Pollock peptides were purified by ultraffltrations,gel filtration,anion-exchange chromatography(Q-FF),and reversed phase high performance liquid chromatography(RP-HPLC) in turn.These results showed that Pollock peptides separated on Q-FF column and three components(B1,B2,B3) were obtained.B2 was identified by RP-HPLC to be homogenous.

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