解脂耶氏酵母URA3基因的敲除

The Disruption of URA3 Gene of Yarrowia lipolytica

DOI：

中文关键词: 解脂耶氏酵母菌 URA3 基因敲除

英文关键词: Yarrowia lipolytica URA3 gene disruption

基金项目:北京市教委科技计划重点项目(KZ200910011001)

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中文摘要:

构建了营养缺陷型解脂耶氏酵母菌株,使之用于遗传标记和高产香味物质γ-癸内酯。作者利用基因同源重组的方法敲除掉尿嘧啶合成酶关键基因URA3基因,用尿嘧啶营养缺陷型培养基(SD-URA)添加一定浓度的5-氟乳清酸(5-FOA)和尿嘧啶筛选获得转化子。实验表明:尿嘧啶营养缺陷型菌株在含有5-FOA和尿嘧啶的培养基上生长而野生型菌株不生长,从而建立了一种快速获得营养缺陷型解脂耶氏菌株的方法。

英文摘要:

In order to get high-yield γ-decalactone and genetic markers,an auxotrophic Yarrowia lipolytica strain was isolated by genetic modification.In this study,URA3,a key gene for uracil synthetase of Yarrowia lipolytica,was disrupted using the method of gene homologous recombination,then transformants were screened by uracil auxotrophic medium(SD-URA) with the combination of 5-FOA and uracil.The results showed that the uracil auxotrophic strain was able to grow at the SD-URA medium containing 5-FOA and uracil while the wild-type strains do not grown.Therefore,an easy and efficient method for obtaining auxotrophic Yarrowia lipolytica strain was established in this study.

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