茶多糖降血糖机制的体外研究

Research on the Mechanism of Tea Polysaccharide Reducing Blood Glucose in Vitro

DOI：

中文关键词: 茶多糖 降血糖 葡萄糖激酶 己糖激酶 机制

英文关键词: tea polysaccharides reducing blood glucose hexokinase glucokinase mechanism

基金项目:国家863计划项目(2007AA100401)

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中文摘要:

通过体外测定不同纯度的茶多糖对羟基自由基和超氧阴离子自由基的清除效果、茶多糖对关键糖代谢酶活性的影响以及茶多糖促进葡萄糖往3T3-L1脂肪细胞转运的能力来研究茶多糖降血糖机制。结果表明纯度高的茶多糖对羟基自由基和氧自由基的清除能力非常低,而纯度低的茶多糖却对这两种自由基有明显的清除效果,对α-葡糖苷酶(葡萄糖淀粉酶、蔗糖酶和麦芽糖酶)活性的抑制作用也非常低,且抑制率与茶多糖的浓度没有明显的相关性,对葡萄糖往外周细胞3T3-L1细胞的转运作用也不明显,但可显著增强葡萄糖激酶和己糖激酶的活性。

英文摘要:

The target of this manuscript is to elucidate the mechanism of TPS reducing blood glucose,for this,the scavenging effect of 89% and 51% tea polysaccharides (TPS) on hydroxyl radical,superoxide radical key carbohydrate metabolic enzymes activities,and the glucose transportation to 3T3-L1 were careful investigated in vitro Results showed that the abilities of scanvenging hydroxyl radical and superoxide radical of TPS with lower purity was abvious,while,that with higher purity was not obvious.The effect on α-glucosidase activities and transportation of glucose to 3T3-L1 cell were also no obvious.However,TPS significantly enhanced the activities of hexokinase(HK) and glucokinase (GK).

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