非水相脂肪酶催化合成L-抗坏血酸硬脂酸酯

Lipase-catalyzed Synthesis of L-ascorbyl Stearate in Non-aqueous Phase

DOI：

中文关键词: 非水介质 脂肪酶催化 L-抗坏血酸硬脂酸酯 合成

英文关键词: non-aqueous medium lipase-catalyzed L-ascorbyl stearate synthesis

基金项目:广东省高校科技成果转化重大资助项目(cgzhzd0805)

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中文摘要:

研究了非水介质中Novozym435脂肪酶催化合成L-抗坏血酸硬脂酸酯(L-AS)。脂肪酶用量固定为L-抗坏血酸质量的20%,底物L-抗坏血酸与硬脂酸的摩尔比限定为1:2,对影响脂肪酶催化的因素如溶剂、温度、溶剂量、反应时间、分子筛用量和摇床转速进行了研究。优化后的反应条件:在30mL叔丁醇中加入6.82mmolL-抗坏血酸,13.64mmol硬脂酸,0.24g脂肪酶和3.0g4A分子筛,摇床转速150r/min,反应在55℃水浴中进行48h。通过分离提纯,产品采用红外及质谱检测后确定为L-抗坏血酸硬脂酸酯,产率最高时达64.7%。脂肪酶重复使用5次,最低产率为61%。

英文摘要:

In this manuscript,the technical conditions of Novozym 435 lipase-catalyzed synthesis of L-ascorbyl stearate (L-AS) in non-aqueous medium were determined with 20% lipase and 1:2 L-ascorbic acid to stearic acid (molar ratio).The optimum reaction conditions listed as follows:30ml t-butanol with 6.82mmol L-ascorbic acid,13.64mmol stearic acid,0.24g lipase and 3.0g 4A molecular sieves,shaker speed 150r/min,the reaction carried out at 55℃ water bath with 48h.Through the separation and purification,the isolated product was finally identified as L-ascorbyl stearate by infrared (IR) and mass spectrometry (MS) detection,the highest yield reached 64.7%.Lipase reused five times,the lowest yield was 61%.

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