法夫酵母产新科斯糖的发酵条件

Studies on Fermentation Conditions for Production of Neokestose by Xanthophyllomyces dendrorhous

DOI：

中文关键词: 法夫酵母 新科斯糖 蔗糖 摇瓶发酵

英文关键词: Xanthophyllomyces dendrorhous neokestose fructo-oligosaccharides submerged fermentation

基金项目:教育部优秀人才计划项目(NCET-07-0379)

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中文摘要:

通过单因素实验及正交试验对法夫酵母摇床发酵产新科斯糖的培养基组成和培养条件进行优化,得出该菌株产新科斯糖的最佳发酵条件为:蔗糖110g/L,玉米浆粉8g/L,种龄48h,接种体积分数10%,发酵周期24h,初始pH6.5,摇床转速220r/min,发酵温度18℃。经优化后新科斯糖产量最高可达73.2g/L,蔗糖利用率为66.5%,比优化前发酵效率提高了87.1%。

英文摘要:

In order to further improve the titer and yield of neokestose production by Xanthophyllomyces dendrorhous,the medium compositions and environmental conditions for neokestose production were optimum by the single factor experiment and the orthogonal experiments,and listed as follows:sucrose 110 g/L,dried corn steep liquor powder 9 g/L,pH 6.5,rotation speed of shaker 220 r/min and inoculation size10% in 25 mL of culture medium in the 250 mL flasks at 18 ℃ for 24 h.The concentration of neokestose by Xanthophyllomyces dendrorhous 269 was achieved at 73.2 g/L,the yield of neokestose on sucrose reached at 66.5%,which was 1.87 times than that of the control.

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