川芎有效成分的体外抗氧化研究

Isolation and Antioxidative Activity of the Substrate Isolated from Chuanxiong

DOI：

中文关键词: 川芎嗪 氧化应激 自由基 血管内皮细胞

英文关键词: tetramethylpyrazine oxidative stress free radical vascular endothelial cell

基金项目:重庆市教育委员会科学技术研究项目资助(NOKJ070304);重庆医科大学人才启动基金资助项目(No0200100263)

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中文摘要:

探讨研究了天然药用植物川芎(Ligusticum chuanxiong Hort.)根茎内有效成分—川芎嗪的体外自由基清除活性,以及对过氧化氢诱导的内皮细胞氧化损伤的抑制作用。流式细胞仪检测川芎嗪对H2O2诱导内皮细胞内活性氧(ROS)产生的抑制作用;Griess法测定川芎嗪对一氧化氮(NO)的清除能力;比色法测定川芎嗪对羟自由基(.OH)、过氧化氢(H2O2)、DPPH自由基(.DPPH)及脂质过氧化的清除能力。川芎嗪可抑制H2O2诱导的内皮细胞内ROS的产生(P

英文摘要:

The objective of this study is to investigate scavenging activity of tetramethylpyrazine(TMP),an effective constituent isolated from the rhizome of nature medicinal plant Ligusticum chuanxiong Hort.,on free radicals in vitro and inhibitory effect of TMP on H2O2-induced oxidative damage of endothelial cells.The inhibition of H2O2-induced intracellular reactive oxygen species(ROS) by TMP was analyzed on a FACS Vantage SE flow cytometer.The suppressive effect of TMP on nitric oxide(NO) production was measured by Griess method.The scavenging effect of TMP on free radicals including hydroxyl radical(·OH),hydrogen peroxide(H2O2),DPPH free radicle(DPPH·) and lipid peroxidation free radical was determined by colorimetry method.It was found that TMP inhibited H2O2-induced production of intracellular ROS and exhibited strong scavenging activity against hydroxyl radical.In addition,TMP displayed scavenging activity against DPPH·,NO,H2O2 and lipid peroxidation.The results presented here indicated that TMP may exhibit scavenging activity on free radicals in vitro and inhibitory effect on oxidative damage of endothelial cells.

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