| 红树林放线菌的筛选与抗真菌和抗肿瘤活性的测定
| The Antifungal and Antitumor Activities of the Isolated Mangrove Forest Actinomycetes
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| DOI:
| 中文关键词: 红树林 放线菌 抗真菌活性 抗肿瘤活性
| 英文关键词: mangrove forest actinomyces antifungal activity antitumor activity
| 基金项目:国家自然科学基金项目(30771646)
| 作者
| 单位
| 李蓉
| 中国海洋大学,食品科学与工程学院,山东,青岛,266003
中国海洋大学,医药学院,山东,青岛,266003
| 张京良
| 中国海洋大学,食品科学与工程学院,山东,青岛,266003
| 江晓路
| 中国海洋大学,食品科学与工程学院,山东,青岛,266003
| 管华诗
| 中国海洋大学,医药学院,山东,青岛,266003
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| 中文摘要:
| 通过从深圳福田红树林根际于不同季节采集的3份土样和2份水样中分离得到95株放线菌,94%的放线菌为链霉菌属。测定95株放线菌发酵液对9种病原真菌的抗菌活性,其中31.6%的放线菌发酵液有不同程度的抗病原真菌作用。同时采用MTT法测定了它们对肺癌细胞A549、白血病细胞株K562两种肿瘤细胞的细胞毒活性,其中21%放线菌发酵液具有细胞毒活性。s33、s62、s65和s68这个4株放线菌发酵液具有抑制5种以上真菌的作用,并且对两种肿瘤细胞株均有作用。
| 英文摘要:
| The obective of this study is to isolate mangrove forest derived actinomyces strains for obtaining the bioactive metabolite producing strains to investigate antifungal and anti-tumor active metabolites.For this,actinomyces strains were isolated by single colony isolation technique.The antifungal activity was assayed by paper method.The anti-tumor activity was assayed by MTT method.Ninety-five strains of actinomyces were isolated from 3 soil samples and 2 water samples collected from a mangrove forest in Futian,south of Shenzhen,China,of which 94% were streptomycetaceae.Nine pathogens were used to test the antifungal activity of the ninety-five strains,among of them 31.6% of the fermentation broth showed the antifungal activity.Two tumor cell lines(A549 and K562) were used to test the antitumor activity with the MTT method.The result indicated that 21% of the fermentation broth presented the antitumor activity.Furthermore,the fermentation broth of the s33,s62,s65and s68 showed antifungal activity toward more than five pathogens and antitumor activity on the two cell lines.
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