食品中百草枯3种检测方法的比较

Comparasion of Three Detection Dethods for Paraquat Residues in Food

DOI：

中文关键词: 百草枯 HPLC,酶联免疫法(ELISA) 胶体金免疫层析发(GICA)

英文关键词: PQ HPLC ELISA GICA

基金项目:国家863计划项目(2007AA10Z428)

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中文摘要:

通过对粮食作物中的百草枯残留检测方法进行比较研究,得到各种方法的优劣。结果:HPLC的最低检测限为12 mg/L,加标回收率为42%～52%,相对标准偏差(RSD)为4.43%;ELISA的最低检测限为0.005 mg/L,加标回收率为70%～80%,相对标准偏差(RSD)为8.15%;GICA的最低检测限为50 mg/L,通过一个标准的颜色反应系列,可以非常简便、快速的对样品进行现场检测,是快速检测发展的一个方向。

英文摘要:

This study investigated the detection methods and the corresponding detection parameters of paraquat(PQ).A serials of results were achieved:(1)The minimum limition of HPLC was 12 mg/L,the recovery ration was 42～52%,the RSD was 4.43%,the precision was very high.(2) the parameters of ELISA were:minimum limitation 0.005μg /L,the recovery ratio 70～80%,RSD 8.15%;.(3)The detection results of GICA is very good,minimum limition 50 mg/L,through a standard color reflected series.Among these detection protocols,GICA method has been proven to be a simple,efficient,quick method to determine the content of PQ in food.

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