混合菌株固态发酵玉米皮生产饲料蛋白

Feed Protein Production from Corn Bran with Mix-Strain Solid-State Fermentation

DOI：

中文关键词: 玉米皮 混菌固态发酵 木聚糖酶 饲料蛋白

英文关键词: corn bran mixed-strain solid state fermentation xylanase feed protein

基金项目:国家自然科学基金青年科学基金项目(20906041)

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中文摘要:

以玉米皮为基本碳源,通过单因素试验和正交试验,对康宁木霉和假丝酵母混合固态发酵生产饲料蛋白进行了研究。以提高木聚糖酶活和真蛋白含量为主要目的,优化得到最佳培养条件为:麸皮25 g/dL,玉米皮75 g/dL,初始固液比为1:1.5,酵母接种体积分数为10%,30℃培养7d。在此条件下,最终得到的产品的木聚糖酶活力为1 300 IU/g左右,真蛋白含量达到20%以上。

英文摘要:

This study was carried out to explore a feed protein production from the corn bran by mixed- stain solid-state fermentation with Trichoderma koningii and Candida tropicalis.With the single-factor and orthogonal experimental optimization of the nutritional and environmental conditions for xylanase activity and protein production,the optimal conditions were as follows: wheat bran 25%,solid-liquid ratio 1:1.5,pH 5.5.Under the above conditions,the true protein content in the final product achieved>20%after the fermentation at 30℃for 7 d,and the xylanase activity reached 1 300 IU/g,which diplayed a considerable potential to future practical application.

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