| 平菇液体菌种摇瓶和发酵罐生长动力学模型的比较
| Comparison of Mycelial Growth Kinetics Model for Pleurotus ostreatus Cultured in Shaking Flask and Fermenter
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| DOI：
| 中文关键词: 平菇 液体菌种 摇瓶 发酵罐 动力学
| 英文关键词: Pleurotus ostreatus liquid spawn shaking flask fermenter kinetics
| 基金项目:河南省科技攻关项目(82102150048)
| 作者
| 单位
| 王兰青
| 河南农业大学,生命科学学院,河南,郑州,450002
| 高玉千
| 河南农业大学,生命科学学院,河南,郑州,450002
| 戚元成
| 河南农业大学,生命科学学院,河南,郑州,450002
| 申进文
| 河南农业大学,生命科学学院,河南,郑州,450002
| 邱立友
| 河南农业大学,生命科学学院,河南,郑州,450002
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| 中文摘要:
| 作者选用玉米粉豆粕粉蔗糖液体培养基,分别采用摇瓶和40 L全自动机械搅拌通风发酵罐培养平菇天达300液体菌种,两种培养方式的菌丝体生长动力学模型均符合Logistic方程。摇瓶培养周期为8 d,最大比生长速率是0.050 h-1,菌丝体质量浓度是1.5001 g/dL。发酵罐培养周期是84 h,最大比生长速率是0.110 h-1,菌丝体质量浓度是1.4750 g/dL。应用发酵罐培养平菇天达300液体菌种更为高效。然而,摇瓶培养的液体菌种菌丝球直径小,单位体积培养液中菌丝球数量是发酵罐的1.4倍,可能与发酵罐培养过程中培养液的粘度下降速度快于摇瓶有关。
| 英文摘要:
| This manuscript study the difference of the fermentation kinetics of Pieurotus ostreatus TD300 in shask and 40 L fermentor.It was found that the mycelial growth kinetics models of both culture modes were accord with Logistic equation.Culturing period,maximum specific growth rate and mycelial concentration cultured in shaking flask were 8 d,0.050 h-1 and 1.5001 g/ml,respectively.While the corresponding value changed to 84 h,0.110 h-1 and 1.4750 g/ml when Pieurotus ostreatus TD300 cultured in fermenter,respectively.The results proven that fermentor culutre was a higher efficiency culture for Pieurotus ostreatus.Furthermore,pellet size and number per unite volume of liquid spawn cultured by shaking flask were smaller and higher than that by fermenter.It was probably caused by the viscosity of medium in shaking flask reduced faster than that in fermentor.
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