壳寡糖修饰多聚半乳糖醛酸酶优化条件研究

Study on Chemical Modification of Polygalacturonase with Chitosan Oligosaccharides

DOI：

中文关键词: 壳寡糖 多聚半乳糖醛酸酶 化学修饰

英文关键词: chitosan oligosaccharide polygalacturonase chemical modification

基金项目:国家自然科学基金项目(10074016)

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中文摘要:

以水溶性低分子量壳寡糖作为修饰剂对已纯化的多聚半乳糖醛酸酶进行化学修饰,通过单因素试验和正交试验探讨pH值、温度、修饰时间、壳寡糖的用量等因素对修饰效果的影响和最佳修饰条件的优化筛选。结果表明,通过正交试验筛选出最佳修饰反应条件为:80mg活化的多聚半乳糖醛酸酶,反应体系中pH为4.0,反应温度为3℃,反应时间为12h,壳寡糖用量为150mg,修饰效果最佳。利用此条件对多聚半乳糖醛酸酶进行化学修饰具有显著的激活作用,修饰后酶的比活力是340.10U/mg protein,比修饰前提高了153.42%。

英文摘要:

Purified polygalacturonase(PG) was chemically modified with water soluble chitosan oligosaccharide(COS) as modifier.The optimum modification conditions were determined by the single factor tests and orthogonal tests and listed as follows: purified PG 80 mg,pH 4.0,temperature 3 ℃,reaction time 12 h,COS content 150mg.Under the optimum conditions,The specific activity of the modified PG was 340.10 U/mg protein,higher 153.42% than that of the unmodified PG.

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