应用酶膜反应器制备酪蛋白生物活性肽

Research of Preparation of Casein Bioactive Peptides Using Enzymatic Membrane Reactor

DOI：

中文关键词: 酶膜反应器 酪蛋白生物活性肽 蛋白质回收率 膜通量 酶活力

英文关键词: enzymatic membrane reactor casein bioactive peptides protein recovery permeate flux enzyme activity

基金项目:国家863计划项目(2008AA10Z313)

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中文摘要:

研究酶膜反应器中超滤压力及循环流速对分离酪蛋白生物活性肽的影响。在截留相对分子质量为10 000切向流超滤酶膜反应器(EMRs)中,采用Alcalase 2.4FG在pH 8.5、温度为50℃条件下对酪蛋白进行连续水解。通过单因素实验研究了操作压力及循环流速对膜通量J、酪蛋白水解度DH、蛋白质回收率S、残余酶活AR、泄漏酶活AF及酶活损失AL等因素的影响,确定最佳的超滤工艺。研究结果表明:增大超滤压力会导致蛋白质回收率的大幅度提高,但会促使酶大量泄漏并对膜造成严重的污染;提高循环流量为140 mL/min时,酶泄漏较少,膜污染程度相对降低,并可获得较高的蛋白质回收率。

英文摘要:

The influence of operating conditions including pressure and recirculation flow rate on the isolation of casein bioactive peptides was investigated in this study.Alcalase 2.4 FG was used to continuously hydrolyse an initial concentration of 6%(w/w) casein at pH 8.5 and 50 ℃ in an enzymatic membrane reactor.The degree of hydrolysis(DH),permeate flux(J),product recovered in permeate(S),enzyme leakage(AF),residual enzyme activity(AR) and enzyme loss(AL) were determined to value the effect of operating conditions of pressure and recirculation flow rate on isolation of casein bioactive peptides by single factor experimental.It showed that a high increased in S with increasing operating pressure,but leading to a serious enzyme leakage and membrane fouling.However,it could reduce enzyme leakage and membrane fouling with a suitable recirculation flow rate of 140 mL/min,meanwhile it could gain a relatively high protein recovery.

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