东部沿海贝类中副溶血弧菌的分布及特征
Distribution and Characterization of Vibrio parahaemolyticus in Shellfish from Eastern Coast of China
DOI：
中文关键词: 副溶血弧菌 血清型 毒力基因 RAPD分型
英文关键词: Vibrio parahaemolyticus serotype,virulence genes,RAPD typing
基金项目:国家863计划项目(2007AA09Z438);青岛市科技计划项目(09-1-3-21-jch)
作者
单位
赵峰
中国水产科学研究院黄海水产研究所,山东青岛266017
上海海洋大学食品学院,上海201306
周德庆
中国水产科学研究院黄海水产研究所,山东青岛,266017
于维森
青岛市疾病预防控制中心,山东青岛,266033
王伟栋
李毅财
刘帅帅
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中文摘要:
为调查我国东部沿海城市零售贝类中副溶血弧菌的分布情况及其血清型和分子特征。共采集了288个贝类样品,采用MPN-PCR方法进行定量研究,分离得到的菌株用RAPD方法进行分子分型并考察其毒力基因携带情况。结果显示,贝类中副溶血弧菌含量季节分布明显,夏季显著高于其他季节(P
英文摘要:
To investigate the distribution,serological and molecular characterization of vibrio parahaemolyticus of shellfish,a total of 288 samples were collected and analyzed in this manuscript.The levels of V.parahaemolyticus were estimated by MPN-PCR and isolated strains were clustered by RAPD analysis.The tdh and trh genes were detected by PCR.The results showed that the distribution of V.parahaemolyticus was disparity in season,the level of V.parahaemolyticus in summer were different from other seasons(P
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