响应面优化绿色木霉菌培养基

Optimization of Medium of Trichoderma viride Using Response Surface Methodology

DOI：

中文关键词: 绿色木霉菌 培养基 菌落生长

英文关键词: Trichoderma viride medium colony growth

基金项目:国家自然科学基金项目(30871765)

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中文摘要:

利用响应面分析法对绿色木霉菌的培养基进行优化。通过测量不同营养条件下绿色木霉菌落生长直径研究其生物学特性,在单因素实验的基础上,选定葡萄糖添加量、丙氨酸添加量和磷酸二氢钾添加量3个因素进行中心组合实验,建立二次回归方程,并应用响应面分析法进行优化。结果表明,绿色木霉菌最佳培养基为葡萄糖2.11 g/dL、丙氨酸0.15 g/dL、磷酸二氢钾0.30 g/dL。在此条件下培养的绿色木霉菌菌落生长直径达到78.00 mm,与预测值77.87 mm接近(CI>95%)。

英文摘要:

In this study,response surface methodology(RSM) was used to optimize the medium compositions for Trichoderma viride growth with the colony growth diameter of T.viride as index.According to the single-factor experiments,three levels of the factors(carbon source addition,nitrogen source addition and phosphorus source addition) were selected for the central composite design to establish a quadric regression equation,and RSM was employed to optimize.The results showed that the optimal conditions for Trichoderma viride were 2.11% glucose,0.15% alanine and 0.3% KH2PO4.Under these conditions,the colony growth diameter of T.viride reached at 78.00 mm,which t was almost equal to the estimated value 77.87mm(CI>95%).

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