不同方法鉴定桑黄真菌的比较

Compare of Different Identifing Methods on Sanghuang

DOI：

中文关键词: 桑黄 形态 rDNA ITS 指纹图谱 鉴定

英文关键词: Sanghuang modality rDNA ITS fingerprint alignment identification

基金项目:四川省自然科学基金青年基金项目(2008ZQ026-072)

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中文摘要:

作者从形态、rDNA ITS分子标记和成分HPLC指纹图谱分析鉴定供试桑黄真菌,从不同水平相互验证鉴定结果。结果表明,形态差异不能作为有效的鉴定证据,成分的HPLC指纹分析构建的系统发育树与基于rDNA ITS序列构建的发育树得到结果基本相符,均淘汰一混淆菌株,而分子标记还将其余菌株鉴定为2类,P.baumii和P.linteus,成分分析没有明显聚类倾向,说明成分分析可以作为菌种(株)鉴定的一种辅助手段,在一定程度上有作用,相对核酸分析而言,不是一个准确可靠的方法。

英文摘要:

In this study,the different identification methods for Sang huang were compared.Sang huang was identified by modality,molecular marker and HPLC fingerprint alignment,and the identifing result was validated one another from different levels.The results showed that(1) the morphological difference of strain could not use as the index for strain identification.(2) the phylogenetic tree from HPLC fingerprint alignmen and from rDNA ITS showed more consistent.(3) molecular marker identified other strains as P.baumii and P.linteus.Based on the above results,the component analysis could regard as assistant means for identifying strains,but was not exact and dependable in relative to nucleic acid analysis.

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