基于SDS增溶的共聚物胶束中辅酶Q10含量分析

Determination of Content of Coenzyme Q10 in Copolymer Micellers with SDS Solubilization

DOI：

中文关键词: 增溶 共聚物胶束 十二烷基硫酸钠 辅酶Q10

英文关键词: solubilization copolymer micelllers sodium dodecyl sulfate coenzyme Q10

基金项目:国家863计划项目(2007AA100403);食品科学与技术国家重点实验室自由探索课题(SKLF-TS200904)

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中文摘要:

对酪蛋白-葡聚糖共聚物胶束作为输送体系而言,所包埋营养素的总量是评价其质量的重要指标。表面活性剂十二烷基硫酸钠（SDS）在将共聚物胶束解体的同时使辅酶Q10增溶于水中,从而便于进行定量分析。实验结果表明:当SDS浓度为0.31 mol/L、孵育温度30℃、硼氢化钠7mg/mL、用量100μL、还原时间35 min时,SDS增溶法的加样回收率在（98.77±1.60）%95.90±0.80%之间。同时,共聚物中酪蛋白单元的内源荧光和外源荧光光谱分析显示,当SDS浓度为00.5 mol/L时,SDS会部分改变酪蛋白单元的疏水结构;且SDS浓度为0.31 mol/L时,SDS与酪蛋白单元疏水区的相互作用几乎不受温度的影响。

英文摘要:

The total content of nutrient is an important index for evaluating the quality of casein-dextran copolymer micellers as a delivery system.Sodium dodecyl sulfate(SDS) as a surfactant can be used to disorganize copolymer micellers and make coenzyme Q10 solubilize in water for quantitative analysis.In this study,the recovery rate of coenzyme Q10 from blank casein-dextran copolymer micellers ranged from(98.77±1.60) % to(95.90±0.80) % with conditions as follows: SDS concentration 0.31 mol/L,incubation temperature 30 ℃,sodium borohydride(7 mg/mL) dL,reduction time 3~5 min.The analysis of synchronous and exogenous fluorescence spectra indicated that the hydrophobic region of casein unit of copolymer was partly changed during solubilization at SDS concentration form 0 to 0.5 mol/L.The interaction between SDS and hydrophobic region was not influenced by temperature when the concentration of SDS was 0.31 mol/L.

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