马氏珠母贝高F值寡肽在Caco-2细胞中吸收的初步研究

Preliminary Study on the Transport of Pinctada Martensii High Fischer Ratio Oligopeptides Across a Caco-2 monolayer

DOI：

中文关键词: 马氏珠母贝 高F值寡肽 Caco-2细胞

英文关键词: Pinctada martensii high Fischer’ ratio oligopeptides Caco-2 cell

基金项目:国家“十一五”支撑计划项目(2007BAD29B09);广东海洋大学引进人才基金项目(0912259)

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中文摘要:

采用Caco-2单层细胞模型体外模拟马氏珠母贝高F值寡肽(PHFP)小肠吸收,研究结果显示:无论是AP面→BL面还是BL面→AP面,PHFP的吸收量均呈增加趋势。添加P-糖蛋白的专属抑制剂维拉帕米后,AP→BL的渗透系数由原来的6.83×10-7cm/min提高到8.16×10-7cm/min,而BL→AP渗透系数由8.84×10-7cm/min降低到2.20 10-7cm/min,说明PHFP溶液在Caco-2细胞中的转运存在外排泵的作用。并且,PHFP在Caco-2细胞转运过程中被细胞中所存在的代谢酶所代谢,而添加维拉帕米后,PHFP的代谢量有所降低。

英文摘要:

The transport of PHFP across a Caco-2 monolayer was studied in this manuscript.The results showed that the absorption quantity of PHFP increased either AP→BL or BL→AP.After adding the inhibitor of P-glycoprotein verapamil,the permeability coefficient(AP→BL) increased from 6.83 ×10-7 cm/min to 8.16 ×10-7 cm/min,and the permeability coefficient(BL→AP) decreased from 8.84×10-7 cm/min to 2.20×10-7cm/min.This results indicated that there existed an efflux system and parts of PHFP were metabolized by metabolic enzymes of cells during the transport,and the metabolism yield of PHFP decreased after adding verapamil.

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