红栓菌液体发酵条件下马钱子中士的宁和马钱子碱的质量分数变化

Determination of the Content of Strychnine and Brucine in Strychnos nux-vomica L.by the Liquid Fermentation with Trametes cinnabarina(Jacq.) Franeh

DOI：10.3969/j.issn.1673-1689.2012.03.012

中文关键词: 马钱子 红栓菌 液体发酵 士的宁 马钱子碱

英文关键词: Strychnos nux-vomica L Trametes cinnabarina(Jacq.) Franeh liquid-state fermentation strychnine brucine

基金项目:江苏省教育厅自然科学基金项目(06KJB360083);江苏省“青蓝工程”项目

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中文摘要:

在单因素实验条件优化的基础上,采用L16(45)正交试验,以士的宁和马钱子碱的质量分数为主要考察指标,结合菌体生物量,研究不同碳源、氮源、装液量、中药量、菌球数量以及发酵时间对红栓菌发酵马钱子的影响,优化红栓菌液体发酵马钱子的最佳培养基和培养条件。优化工艺为:以麦芽糖为碳源,豆饼粉为氮源,装液量为100mL(250mL的三角瓶),加入0.25g马钱子细粉,接入3个大小均一的红栓菌菌球,28℃摇床培养5d。与发酵空白比较,红栓菌-马钱子发酵液中士的宁和马钱子碱的质量分数总和降低了8.74%,菌体生物量达10.6g/L,增加37.7%,同时马钱子碱氮氧化物和士的宁氮氧化物的质量分数增加。马钱子能促进红栓菌菌体的生长,液体发酵培养可达到降低马钱子中毒性成分质量分数的目的。

英文摘要:

Based on the results of the single factor(carbon and nitrogen sources),the effect of broth content,mycelial pellet amount,powder weight and fermentation time on alkaloids content and biomass growth were further discussed by using L16(45) orthogonal test.The optimized culture conditions were acquired and listed as follows: maltose is carbon source,soybean cake powder is nitrogen source and liquid volume of 100 mL(a 250 mL flask),by adding 0.25 g powder,inoculated three evenly sized mycelial pellet,cultured at 28 degrees celsius for 5 days.After optimization the total content of strychnine and brucine in the culture medium reduced 8.74% compared with the control.Biomass could reach 10.6 g/L.Its rising rate was 37.7%.Meanwhile strychnine N-oxide and brucine N-oxide increased.The results showed the drug could promote the fungal growth and it can decrease the content of toxic constituents by liquid fermentation.

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