三角帆蚌多糖制备及基本理化性质

Preparation and Preliminary Feature of Polysaccharides from Hyriopsis cumingii

DOI：10.3969/j.issn.1673-1689.2011.01.013

中文关键词: 三角帆蚌 多糖 制备

英文关键词: Hyriopsis cumingii polysaccharides preparation

基金项目:安徽省高等学校自然科学研究项目(KJ2010B256)

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中文摘要:

为充分利用三角帆蚌资源、开发利用其中的多糖类物质,运用热水浸提、乙醇沉淀、Sevag法脱蛋白质、乙醇再沉淀的方法提取获得了三角帆蚌多糖(HCPS),提取率为3.5%左右。运用DEAE-纤维素-52色谱和葡聚糖G-100色谱对三角帆蚌粗多糖进行分离纯化,得到3个纯化组分(HCPS-1、HCPS-2和HCPS-3),3个组分HCPS-1、HCPS-2和HCPS-3的得率分别是26.9%、30.2%和3.9%。运用化学的、物理的方法测量分析了三角帆蚌粗多糖及其纯化组分的部分理化性质,包括总糖质量分数、蛋白质质量分数、硫酸基质量分数、相对粘度、单糖组成、相对分子质量以及红外光谱(FTIR)。HCPS-3具有较高的蛋白质质量分数、较高的硫酸基质量分数和复杂的单糖组成,这些与HCPS-1、HCPS-2明显不同。

英文摘要:

In order to utilize polysaccharides resources in Hyriopsis cumingii,polysaccharides from Hyriopsis cumingii(HCPS) was extracted by using methods of hot water lixiviation,ethanol precipitation,Sevag’s deproteination and ethanol precipitation again,the extraction yield of crude HCPS was about 3.5%.The crude HCPS was separated firstly through DEAE-cellulose 52 chromatography column and further purified through chromatography column of Sephadex G-100.Three purified fractions,named as HCPS-1,HCPS-2 and HCPS-3,were obtained.The recovery rates of HCPS-1,HCPS-2 and HCPS-3 was 26.9%,30.2% and 3.9% respectively.Preliminary feature of crude HCPS,HCPS-1,HCPS-2 and HCPS-3,including polysaccharides content,protein content,sulfuric radical content,relative viscosity,monosaccharide composition,relative molecular weight and FTIR spectrum,were determined.Results showed that HCPS-3 had more sulfuric acid radicals and protein than HCPS-1 and HCPS-2.The monosaccharide composition of HCPS-3 was more complicated than that of HCPS-1 or HCPS-2.HCPS-3 was quite different from HCPS-1 or HCPS-2.

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