响应面法优化康宁木霉产纤维素酶固态发酵培养基

Optimization of Trichoderma konigii Cellulase Production on Solid-State Fermentation Medium by Response Surface Analysis

DOI：10.3969/j.issn.1673-1689.2011.01.019

中文关键词: 康宁木霉 固态发酵 纤维素酶 响应面法 培养基优化

英文关键词: Trichoderma konigii solid state fermentation cellulase response surface analysis medium optimization

基金项目:国家自然科学基金青年科学基金项目(20906041)

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中文摘要:

采用响应面法试验设计,对康宁木霉(Trichoderma konigii)固态发酵玉米皮生产纤维素酶的条件进行了优化,并建立了纤维素酶随麸皮添加量、物料初始水分质量分数和pH值变化的二次回归方程。利用该方程探讨了各因子对纤维素酶的影响。结果表明,各因子对纤维素酶的影响顺序为:物料初始水分质量分数>麸皮添加量>pH值,各因子间交互作用不显著。结合单因素实验,最终确定适宜的发酵条件为:麸皮添加量24.4 g/dL;营养液添加量:1.0 g/dL硫酸铵,0.05 g/dL磷酸二氢钾,0.1 g/dL硫酸镁,0.2 g/dL乳糖;初始水分质量分数58.6%;pH 5.5。在此条件下发酵120 h,滤纸酶活达到11.3 IU/g,较未优化前提高了2.9倍。

英文摘要:

Corn bran was used as the substrate for cellulase production using Trichoderma konigii to achieve its high value utilization,and the nutritional and envionmental conditions were optimized for enhancing cellulase yield.Such key parameters as wheat bran addition,initial moisture content and pH were optimized by Box Behnken experimental design.Experimental results showed that the impact order of three key parameters on cellulase production ware listed as follows: initial moisture content > wheat bran addition > pH,while the dependence between three factors was not significant.Predicted results indicated that cellulase production was high up to 11.3 FPAase units / g dry substrate after the fermentation of 5 days with 24.4% of wheat bran addition,1.0% of ammonium sulfate,0.05% of potassium dihydrogen phosphate,0.1% of magnesium sulfate,0.2% of lactose,58.6% of the initial water content and pH5.5.

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