红树林土壤纤维素酶产生菌筛选及产酶条件研究

Screening of Cellulase-Producing Strains from Mangrove Soil and Optimization of Fermentation Conditions

DOI：10.3969/j.issn.1673-1689.2011.01.025

中文关键词: 红树林 纤维素酶产生菌 菌种筛选 产紫青霉 发酵条件

英文关键词: mangrove cellulase-producing strain strain screening Penicillium purpurogenum fermentation conditions

基金项目:海南大学博士启动基金资助项目(Rndy0706)

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中文摘要:

采用CMC唯一碳源平板法和内切葡聚糖酶、外切葡聚糖酶和β-葡萄糖苷酶等3种酶平板鉴别法从海南红树林土壤中分离到109个有阳性信号的菌株,经发酵产酶复筛选出一株产纤维素酶活相对较高的真菌HBZ003。经鉴定该菌为产紫青霉(Penicillium purpurogenum)。通过发酵产酶条件优化,获得最佳培养基组成为:麸皮8 g/L,CMC 2 g/L,(NH4)2SO43 g/L,KNO32 g/L,KH2PO43 g/L,NaCl 6 g/L,CaCl20.5 g/L;发酵条件为250mL三角瓶中装培养液100mL,在pH4.0、30℃,160 r/min条件下振荡培养5 d,测得发酵液中CMCase和FPA分别为16.04U和4.08 U。

英文摘要:

In this study,109 strains with positive signals were collected and screened from mangrove soil using unique C-source plating and differential plating technique for Endo-glucanase,exo-glucanase and β-glucosidase.A fungus HBZ003 with high enzyme activity was obtained identified as Penicillium purpurogenum.The optimum medium components for cellulose production by strain HBZ003 were determined and listed as follows: wheat bran 8 g/L,CMC 2 g/L,(NH4)2SO4 3 g/L,KNO3 2 g/L,KH2PO4 3 g/L,NaCl 6 g/L and CaCl2 0.5 g/L.The maximum activities of carboxymethyl cellulase activity(CMCase) and filter paper activity(FPA) were reached at 16.04U and 4.08 U,respectively,after culture 5 days under 30 ℃,initial pH 4.0 and 160 r/min.

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