碱性蛋白酶产生菌的筛选鉴定及培养优化

Isolation and Identification of the Alkaline Protease-Producing Bacteria and Optimization of Its Culture Conditions

DOI：10.3969/j.issn.1673-1689.2012.12.013

中文关键词: 碱性蛋白酶 16S rDNA 解淀粉芽胞杆菌 筛选鉴定 发酵优化

英文关键词: alkaline protease 16S rDNA Bacillus amyloliquefaciens isolation and identification optimization

基金项目:山东省高等学校科技计划项目(J12LD02);鲁东大学科研基金项目(LY20083305)

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中文摘要:

通过对筛选的产碱性蛋白酶菌株进行形态观察、生理生化试验和16S rDNA序列分析鉴定,确定高活力菌株为解淀粉芽孢杆菌。发酵碳源、氮源和金属盐的优化,确定优化培养组分。对接种体积分数、培养温度和培养pH进行研究,确定最佳培养条件。在最优条件下培养18 h后获得最大酶活力为395.18 U/mL,产酶能力比优化前提高了2.55倍。该研究为深入研究工业发酵生产碱性蛋白酶提供了参考。

英文摘要:

Research of screening and fermentation about Bacillus sp.as the protease producing strain is extremely necessary due to their characteristics of heat and pH stability.In this study,strain with producing alkaline protease was screened from the offshore soil of Yantai and the morphological,physiological and biochemical characteristics as well as 16S rDNA sequence homology of the isolation were studied.From the above characteristics,the strain was identified as Bacillus amyloliquefaciens.For industrial production of protease,the optimum nutrition and environmental conditions were determined.Under the optimum conditions,the maximum protease activity reached at 395.18 U/mL at 18 h,which was about 2.55 fold higher than that of the the original medium.The study provides reference data for further industrial fermentation production of alkaline protease.

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