桦褐孔菌总黄酮体内外抗氧化作用

Study on Antioxidation Effect in Vitro and in Vivo of Total Flavonoids from Inonotus obliquus

DOI：10.3969/j.issn.1673-1689.2012.10.009

中文关键词: 桦褐孔菌 总黄酮(TFIO) 自由基 抗氧化活性

英文关键词: Inonotus obliquus total flavonoids(TFIO) free radical antioxidant activity

基金项目:福建省自然基金重点项目(2009N0049);福建省教育厅JA类项目(JA11352);泉州师范学院学科带头人培养基金项目

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中文摘要:

研究了桦褐孔菌总黄酮(TFIO)的体内外抗氧化功效。采用DPPH.自由基体系,脂肪氧合酶体系评价TFIO的自由基清除能力与脂肪氧合酶活力抑制作用;用分光光度法测定TFIO对H2O2诱导小鼠氧化溶血的抑制作用;并用丙二醛(MDA)及活性氧(ROS)试剂盒测定TFIO体内外作用对小鼠肝组织MDA及ROS含量的影响。体外实验发现:4.00 mg/mL TFIO对DPPH.清除率达76.77%,对脂肪氧合酶活抑制率达81.73%,对H2O2诱导的小鼠红细胞(RBC)溶血抑制率为83.33%,对小鼠体外肝匀浆MDA生成的抑制率分别为36.73%(非诱导组)、20.34%(Fe2+诱导组)与48.59%(H2O2诱导组),并使体外肝匀浆抗活性单位极显著增加至50.75 U/mL(P

英文摘要:

To investigate the antioxidant effects in vitro and in vivo of Inonotus obliquus total flavonoids(TFIO),the radical scavenging capacity of TFIO and its inhibiting effect on lipoxidase activity were evaluated by the DPPH· model and the lipoxidase system,respectively.Meanwhile,the inhibitory action on hemolysis of mice RBC(red blood cell) by H2O2-induced was detected by spectrophotometry method,the effects in vitro and in vivo of TFIO on the formation of MDA(malondialdehyde) and ROS(reactive oxygen species) in mice liver were detected by the reagent kits.The results showed that in vitro,4.00 mg/mL TFIO showed 76.77% scavenging rate on DPPH·radical,81.73% inhibiting rate on lipoxygenase,83.33% inhibitory rate on mice RBC hemolysis by H2O2-induced,36.73%(non-induced),20.34%(Fe2+-induced) and 48.59%(H2O2-induced) inhibiting rates on MDA formation in mice liver homogenate,respectively,it also significantly increased the anti-ROS unit of mice liver homogenate to 50.75 U/mL(P

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