大麦芽多糖的降血糖活性及结构解析

Hypoglycemic Effect and Structural Determination of Polysaccharides from Barley Malt

DOI：10.3969/j.issn.1673-1689.2012.10.013

中文关键词: 大麦芽 多糖 降血糖作用 结构

英文关键词: barley malt polysaccharides hypoglycemic effect structure

基金项目:国家“十一五”科技支撑计划项目(2007BAD83B02)

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中文摘要:

用四氧嘧啶糖尿病小鼠模型,考察大麦芽粗多糖的降糖作用,并通过DEAE-cellulose、HW-55F、Sephacryl S-400柱对有降血糖活性的组分进行分离。采用苯酚-硫酸法、薄层色谱法检测多糖纯度,通过单糖组成分析和1H-NMR、13C-NMR、135DEPT-NMR检测对多糖初级结构进行表征。结果表明:大麦芽粗多糖具有降低小鼠空腹血糖作用,对其餐后血糖效果不显著;粗多糖经DEAE-cellulose柱分离得到中性多糖和酸性多糖两个部分,其中水洗中性多糖经过HW-55F、Sephacryl S-400柱层析得到大麦芽多糖BPⅠ和BPⅡ。BPⅠ结构由呋喃葡萄糖组成,以1→3键相连,α和β构型交替出现;BPⅡ为半乳糖分子以1→4键相连形成主链,另有一个半乳糖分子连在主链糖分子的6-位上,形成侧链。其中,BPⅡ在大麦芽中首次报道。

英文摘要:

Purpose: To investigate the separation method and the structure of polysaccharides with hypoglycemic effect from barley malt(BP).Methods: Alloxan-induced diabetic mice model was built,in which the hypoglycemic effect of polysaccharides from barley malt were determined.The polysaccharides from barley malt was purified to homogeneity through DEAE-cellulose,HW-55F and Sephacryl S-400 column chromatography.Meanwhile,the purity identification was analyzed by phenol-sulfuric method and thin layer chromatography.The structure was investigated using sugar composition analysis and 1H-NMR,13C-NMR,135DEPT-NMR analysis.Results: The polysaccharides can decrease fasting plasma glucose of the diabetes mice efficiently,and showed no significant effect on postprandial plasma glucose.Neutral polysaccharides(NBP)and acidic polysaccharides was isolated by DEAE-cellulose.BPⅠ and BPⅡ were further isolated from NBP by HW-55F and Sephacryl S-400.BPⅠconsists only of glucofuranose units joined by 1→3 bond,and both of α and β-configuration appear alternately.The main chain of BPⅡ is galactoses linked by 1→4 bond,and the galactose,forming lateral chain,is linked to C-6 of galactose in the main chain.BPⅡ is reported for the first time from barley malt.

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