中草药提取物抑制H_2O_2诱导人皮肤细胞凋亡的初步研究

Inhibitory Effect of Chinese Herbs Extracts on Human Dermal Fibroblasts Apoptosis Induced by H2O2

DOI：10.3969/j.issn.1673-1689.2012.09.003

中文关键词: 人皮肤成纤维细胞 H2O2 细胞凋亡 中草药

英文关键词: human dermal fibroblast H2O2 apoptosis Chinese herbs

基金项目:国家自然科学基金项目(20871059)

摘要点击次数: 192

全文下载次数: 690

中文摘要:

建立了过氧化氢(H2O2)诱导人皮肤成纤维细胞损伤及凋亡模型,并研究几种具有抗氧化作用的复方中草药提取物对细胞损伤及凋亡的影响。采用MTT、Hoechst染色及TUNEL等方法检测过氧化氢诱导细胞后以及加入中草药提取物后细胞的存活率、细胞形态以及细胞凋亡的状况。实验结果显示:在浓度为0.8 mmol/L,作用时间6 h,过氧化氢能诱导人皮肤成纤维细胞损伤和凋亡(p

英文摘要:

This study aims to establish the cell injury and apoptosis model of human dermal fibroblasts induced by hydrogen peroxide in vitro and investigate the protective effects of several Chinese herbs on hydrogen peroxide-induced oxidative stress injury.The viability of human dermal fibroblasts was measured with MTT chromatometry,then the morphological changes of cells were observed through Hoechst 33342 staining and the apoptotic cells were detected by TUNEL assay.The results showed that normal human dermal fibroblasts were damaged by hydrogen peroxide at a appropriate concentration(0.8 mmol/l) for 6 h.The results also indicated that Chinese herbs increased obviously the cell viability and decreased the apoptosis rate.It was concluded that Chinese herbs could protect human dermal fibroblasts from hydrogen peroxide-induced oxidative stress injury.

查看全文 查看/发表评论 下载PDF阅读器