水杨酸对中国红豆杉内生真菌代谢产紫杉醇的影响

Effects of Salicylic Acid on Paclitaxel-Producing Fungal Endophytes of Taxus chinensis

DOI：10.3969/j.issn.1673-1689.2012.09.008

中文关键词: 水杨酸 中国红豆杉 内生真菌 紫杉醇

英文关键词: salicylic acid Taxus chinensis endophytic fungi paclitaxel

基金项目:陕西省教育厅重点实验室科研专项项目(2010JS045);陕西省教育厅科研专项项目(2010JK461)

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中文摘要:

在中国红豆杉内生真菌液体发酵培养的不同阶段,添加不同浓度的水杨酸(SA),研究其对中国红豆杉内生真菌菌丝体及代谢产紫杉醇含量的影响。结果表明,在中国红豆杉内生真菌稳定期初期15 d时添加5.0 mg/L水杨酸,内生真菌产紫杉醇的量为604.0μg/L。且不同质量浓度的水杨酸均可提高紫杉醇的产量,其中25 mg/L水杨酸对紫杉醇合成的效果最为明显,紫杉醇质量浓度为890.0μg/L,是对照组的1.88倍。

英文摘要:

In this paper,the effects of salicylic acid(SA) on paclitaxel metabolism of endophytic fungus from Taxus Chinensis was preliminary studied.SA was added to the fermentation broth with different concentrations at different time.The results showed that SA at 5.0 mg/L can improve the yield of paclitaxel to 604.0 ug/L at the beginning of the stabilization period of the endophytic fungus.And different concentrations of SA can also improve the yield of paclitaxel as the same,while SA at 25 mg/L is the most obvious,the yield can reach to 890.0 ug/L.It’s 1.88 times to the fermentation broth which SA not added.

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